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SHC014
MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA
Contains a gene encoding TurboGFP, under the control of the UbC promoter
Synonym(e):
MISSION® Control Vectors
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Über diesen Artikel
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Segment
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The UbC-TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.
Silencing of the CMV promoter can be a problem in some cell types. For these cells, the ubiquitin promoter (UbC) can be a viable alternative. The MISSION UbC-TurboGFP Control Vector is a 9092 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP, under the control of the UbC promoter. The UbC-Turbo-GFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones for applications in which an alternate promoter is necessary.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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Dieser Artikel | |||
|---|---|---|---|
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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