The transduction efficiency increase observed when using the ExpressMag products will depend on the cell line. It is reasonable to expect a roughly 10-fold or greater increase.
SHM01
MISSION® ExpressMag® Supermagnet-Kit
Increases transduction efficiency
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Unterstützung erhaltenproduct line
MISSION®
shelf life
≥2 yr at ~4 °C
storage temp.
2-8°C
General description
To view protocols from Sigma collaborators for ExpressMag system visit the link below.
www.sigmaaldrich.com/mission-protocols
www.sigmaaldrich.com/mission-protocols
Application
MISSION® ExpressMag® Super Magnetic Kit contains all of the components needed to start enhancing your lentiviral transductions. The kit contains a 100 μL vial of ExpressMag™ Beads, enough for 63 wells of a 96 well plate. These beads bind the lentiviral particles and enable the ExpressMag™ magnetic plate to pull the virus down to the surface of cells thereby significantly increasing transduction efficiency. This product can be used in conjunction with standard sized well plates, T-25 through T-75 tissue culture flasks, and up to 5 cm dishes. The kit has been documented to enhance MISSION® lentiviral transduction of suspension 293T as well as allowing the transduction of primary human cells such as keratinocytes at MOI as low as 1. See the technical bulletin for other cell lines transduced with the ExpressMag™ system.
Legal Information
ExpressMag is a registered trademark of Sigma-Aldrich Co. LLC
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Do not autoclave! Clean surface of magnet with 70% isopropyl alcohol.
Magnets should be kept away from magnetic storage devices, such as hard disks, ID cards, and credit cards, and from electronic devices and ferromagnetic materials. Persons with cardiac pacemakers should not work with these magnets.
Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artikel
Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Protokolle
Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.
FACS sorts cells based on light scattering and fluorescence for objective cell analysis.
This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.
Verwandter Inhalt
Instructions
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What is the typical fold increase in transduction efficiency when using ExpressMag beads?
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What other viruses have been successfully introduced into cells via the use of ExpressMag beads?
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Adenovirus, SIV, Retrovirus, Measles virus and HIV have all been successfully introduced into cells using ExpressMag.
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What MISSION products have been successfully introduced into cells using ExpressMag® beads?
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MISSION Lentiviral Particles (various targets/clones), SHC002V, SHC003V and SHC008V have been successfully introduced into cells using ExpressMag beads.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What cell lines have been transduced successfully using ExpressMag Beads?
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The following cell types have been successfully transduced using ExpressMag Beads: PBL, PBMC, T Cells, GMB primary tumor cells, A549, NYGM, T98G, U251, U87,YH-13, YK6-1, KS-1, NIH3T3, K562, Vero, HeLa, CHO, L929, B95a, H9, HUVEC, CD4+ Primary Human T-Cells, Human Bone Marrow Macrophages, Primary Keratinocytes, SKOV3, MM-AN, MEF and CD 34+.
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