Yes, this product can be ordered with a higher volume and/or titer. A custom order will be required for this request.
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SHC003V
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles
Green fluorescent protein marker to monitor transduction efficiency
Synonym(e):
MISSION®, MISSION® TurboGFP™ Control Transduction Particles
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Über diesen Artikel
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x106 VP/ml (via p24 assay)
Technique(s):
capture ELISA: 106 TU/mL using p24
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenQuality Segment
product line
MISSION®
concentration
≥1x106 VP/ml (via p24 assay)
technique(s)
capture ELISA: 106 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
General description
This construct aids in interpretation of experimental design and results by providing a green fluorescent protein marker for monitoring success in transduction of your cells of interest.
TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from the copepode Pontellina plumata. The TurboGFP transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-CMV-TurboGFP (SHC003). It is a positive control to monitor transduction efficiency.
Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Transduction Particles has been used to transduce HCT116 (human colon carcinoma) cell line for 2D culture and to form 3D spheroids. It has also been used to generate fluorescent cell lines by transduction.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboGFP is a trademark of Evrogen Co.
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Dieser Artikel | |||
|---|---|---|---|
| description Green fluorescent protein marker to monitor transduction efficiency | description Contains a gene encoding TurboGFP, under the control of the UbC promoter | description Contains a gene encoding TagRFP | description shRNA sequence targeting tGFP |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) capture ELISA: 106 TU/mL using p24 | technique(s) capture ELISA: 106 TU/mL using p24 |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −70°C | storage temp. −70°C | storage temp. −70°C | storage temp. −70°C |
| Quality Level 100 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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いつもお世話になっております。 SHC003Vの購入を検討しております。 この商品のレンチウイルス量に関して、10^6 TU/mLで200uL以外の力価、量は選択できるのでしょうか。 お答えいただけると幸いです。
1 answer-
Helpful?
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このレンチウイルス液の液量は何uLでしょうか？ 教えて頂けると幸いです。
1 answer-
As listed in the 'DESCRIPTION' section under 'General Description', this product is supplied as a 200 µL frozen stock containing at least 10^6 TU/ml. Please see the link below to review additional infomation available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/791/422/shc003vbul.pdfHelpful?
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