RAB0352

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr²⁰²/pTyr²⁰⁴), phospho-JNK (pThr¹⁸³/pTyr¹⁸⁵), and phospho-p38 MAPK (pThr¹⁸⁰/pTyr¹⁸²) in cultured cell lines

192 assays

• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... MAPK1(5594), MAPK14(1432), MAPK3(5595), MAPK8(5599) ; mouse ... MAPK1(26413), MAPK14(26416), MAPK3(26417), MAPK8(26419) ; rat ... MAPK1(116590), MAPK14(81649), MAPK3(50689), MAPK8(116554)
• Species reactivity: mouse, human, rat
• Storage temp.: −20°C
• Shipped in: wet ice

Eigenschaften

• usage: 192 assays
• species reactivity: mouse, human, rat
• technique(s): ELISA: suitable, capture ELISA: suitable
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... MAPK1(5594), MAPK14(1432), MAPK3(5595), MAPK8(5599) ; mouse ... MAPK1(26413), MAPK14(26416), MAPK3(26417), MAPK8(26419) ; rat ... MAPK1(116590), MAPK14(81649), MAPK3(50689), MAPK8(116554)

Beschreibung

General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵) and p38 MAPK (pThr^180m / pTyr¹⁸²) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr²⁰² / pTyr²⁰⁴), JNK (pThr¹⁸³ / pTyr¹⁸⁵), p38 MAPK (pThr¹⁸⁰ / pTyr¹⁸²) synthetic phosphopeptides

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit-Komponenten auch einzeln erhältlich

• RABBLOCK 5x Blocking Solution (Item F)
• RABFIX1 Fixing Solution (Item D)
• RABHRP1 HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1)
• RABHRP2 HRP-conjugated Anti-Mouse IgG Concentrate (Item I2)
• RABPLATE1 Uncoated Microplate
• RABQUENCH Quenching Solution for Cell-based ELISA Assay (Item E)
• RABSTOP1 Stop Solution
RABTMB1 TMB Substrate Reagent (Item J)
RABWASH1 20X Wash Buffer Concentrate A
RABWASH2 20X Wash Buffer Concentrate B

Alle anzeigen (10)

Sicherheitshinweise

• pictograms: GHS08,GHS05,GHS07
• signalword: Danger
• hcodes: H290,H302 + H332,H315,H317,H318,H335,H341,H350,H412
• pcodes: P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3