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P5538
Phosphoglucose-Isomerase aus Bacillus stearothermophilus
lyophilized powder, 300-1,000 units/mg protein
Synonym(e):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 units | Warenkorb auf Verfügbarkeit prüfen | € 397,00 € 337,45 |
250 units | Warenkorb auf Verfügbarkeit prüfen | € 832,00 € 707,20 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-603-1
MDL number:
Specific activity:
300-1,000 units/mg protein
€ 337,45
Listenpreis€ 397,00Sparen Sie 15%Aktion nur im Web
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Technischer Dienst
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Unterstützung erhaltenform
lyophilized powder
Quality Segment
specific activity
300-1,000 units/mg protein
mol wt
189 kDa
composition
Protein, ≥60% biuret
storage temp.
−20°C
General description
The enzyme is part of the glycolytic pathway. Also, it is important in the industrial production of fructose 1,6-diphosphate (FDP) from glucose. The molecular mass is found to be approximately 189 kDa and it consists of four subunits, each with a molecular mass of approximately 50 kDa. Optimum pH is found to be between 9-10 and the isoelectric point is 4.2.
Application
Phosphoglucose Isomerase (PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis. It is highly conserved in bacteria and eukaryotes. It is used in sugar assays to convert fructose to glucose. This product is from Bacillus stearothermophilus.
The enzyme from Sigma has been used in the determination of fructose 6-phosphate in a mutant strain of Rhizobium meliloti.[1]
Biochem/physiol Actions
Phosphoglucose Isomerase fuctions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.
Physical form
lyophilized powder containing Tris buffer
Other Notes
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 9.0 at 30 °C.
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Dieser Artikel | |||
|---|---|---|---|
| specific activity 300-1,000 units/mg protein | specific activity ≥200 units/mg protein | specific activity ≥400 units/mg protein (biuret) | specific activity ≥3,000 units/mg protein (biuret) |
| form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 189 kDa | mol wt - | mol wt tetramer 119,500 Da±600 | mol wt - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| composition Protein, ≥60% biuret | composition protein, 70-100% biuret | composition - | composition Protein, ≥20% |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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