This product is intended for research use only. Unless otherwise stated this product is not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Fortfahren mit
P3288
Phosphatpuffer pH-Wert 7,2 bei 25 °C
for hematology and histology staining techniques
Synonym(e):
Phosphatpuffer
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 vial | Warenkorb auf Verfügbarkeit prüfen | € 110,00 |
12 vials | Warenkorb auf Verfügbarkeit prüfen | € 149,00 |
Über diesen Artikel
NACRES:
NA.47
UNSPSC Code:
12352107
Technischer Dienst
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Unterstützung erhaltenQuality Segment
form
powder
color
white
pH
7.10-7.30
application(s)
hematology
histology
storage temp.
room temp
General description
Phosphatpuffer ist die am häufigsten verwendete Komponente von Spüllösung während chirurgischen Eingriffen, um lebendes Gewebe zu erhalten. Er wird auch in immunhistochemischen Färbungsverfahren eingesetzt. Phosphatpuffer dient als wichtiges Fixiermittel in der Licht- und Elektronenmikroskopie. Seine Pufferkapazität ist pH-sensitiv und wird bevorzugt bei einem physiologischen pH-Wert (7,2–7,4) verwendet.
Application
Zur Verwendung als Puffer in verschiedenen Färbungsverfahren nach Romanowsky vorgesehen, einschließlich Wright-Färbung, Wright-Giemsa, Giemsa, May-Grünwald, Jenner und Leishman. Phosphatpuffer, pH-Wert 7,2 bei 25 °C wird zur Entnahme von Oozyten aus Schweineovarien eingesetzt.
Preparation Note
Jedes Fläschchen erzeugt 3,8 l Puffer, 8,3 mM.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level 500 | Quality Level 200 |
| form powder | form powder | form powder | form liquid |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) - |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| pH 7.10-7.30 | pH 6.60-6.80 | pH 7.60-7.80 (25 °C) | pH 7.2-7.6(1x solution) |
| color white | color white | color - | color - |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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The current lot, 0000447969, in our lab states that this product is for R&D use only. Has this product been cleared for clinical use?
1 answer-
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could you provide me with the Wright-Giemsa protocol referenced in the question above about how to solubilize Product P3288, Phosphate Buffer pH 7.2. We need official instructions for use and the product did not come with it.
1 answer-
If this product is ordered separately, no instructions are provided. Instructions for preparing P3288 are found in the WG Instructions for Use that ship with each bottle of WG16, WG32, WG80, or WG128. Below is the information from the Reagent section of the WG procedure Instructions For Use
The phosphate buffer should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Discard the working phosphate solution if turbidity or visible bacterial growth is present. Store the working phosphate buffer solution at 2-8°C
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Once made how long is the solution good for?
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The long term stability of this product in solution has not been evaluated. Upon reconstitution, the solution should be observed for any particulate matter or turbidity which would indicate possible microbial growth. However, similar prepared solutions such as P3619, a 1M Phosphate Buffer, are not assigned an expiration date. These products are quite stable and offer a warranty of 1 year from the date of shipment.
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How to solubilize Product P3288, Phosphate Buffer pH 7.2?
1 answer-
The instructions for reconstitution of Product P3288, Phosphate buffer pH 7.2 at 25 °C are indicated on the Wright-Giemsa protocol (see attached packaging insert).Briefly, Product P3288, Phosphate buffer pH 7.2 should be prepared by diluting 1 vial of buffer to 1 gallon or 3.8 liters of deionized water. Mix well to dissolve. Store Phosphate Buffer at room temperature (18-26°C). Store working phosphate buffer solution at 2-8°C. Discard the working phosphate solution if turbidity or visible bacterial growth is present.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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