P0515

Phospholipase D from Arachis hypogaea (peanut)

Type II, lyophilized powder, ≥60 units/mg protein

• Synonym(e): Lecithinase D, Phosphatidylcholine phosphatidohydrolase
• CAS-Nummer: 9001-87-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-639-8
• MDL number: MFCD00131897
• EG-Nummer: 3.1.4.4 (BRENDA, IUBMB)
• Specific activity: ≥60 units/mg protein
• Biological source: Arachis hypogaea

Eigenschaften

• biological source: Arachis hypogaea
• Quality Segment: 200
• type: Type II
• form: lyophilized powder
• specific activity: ≥60 units/mg protein
• composition: Protein, ~30%
• storage temp.: −20°C

Beschreibung

General description

Phospholipase D is a phospholipid hydrolyzing enzyme and an important component of receptor-mediated signal transduction responses and regulated secretion.

Phospholipase D (PLD) is abundantly found in the eukaryotes and prokaryotes. It is present in all mammalian cells. In mammals, PLD is initiated by various hormones, neurotransmitters, growth factors and cytokines.

Application

Research has shown ADP-ribosylation factor regulation of phospholipase D is important in the release of nascent secretory vesicles from the trans-Golgi network. It has also been used in a study to investigate stimulation of Na⁺-Ca²⁺ exchange activity in canine cardiac sarcolemmal vesicals.

Phospholipase D from Arachis hypogaea (peanut) has been used in the preparation of lipase enzyme solution. It has also been used to determine its influence on human neutrophil respiratory function.

Biochem/physiol Actions

Phospholipase D is involved in conferring drought susceptibility in peanuts, which increases the risk of aflatoxin contamination.

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

Phospholipase D (PLD) modulates cell growth, secretion and the actin cytoskeleton.

Physical form

Partially purified, lyophilized powder containing buffer salts

Analysis Note

Protein determined using biuret, unless otherwise indicated.

Other Notes

One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• ppe: Eyeshields, Gloves, type N95 (US)