The Cartagena Protocol is not applicable to this product. While the manufacuturing process is proprietary, current methods of extraction and purification would eliminate the presence of any whole, vialble organisms. This is not a sterile product. Prepared solutions may be filter-sterilized prior to use.
Fortfahren mit
P4912
Phospholipase D from Streptomyces sp.
Type VII, lyophilized powder, ≥150 units/mg solid
Synonym(e):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
250 units | Warenkorb auf Verfügbarkeit prüfen | € 276,00 |
500 units | Warenkorb auf Verfügbarkeit prüfen | € 463,00 |
1000 units | Warenkorb auf Verfügbarkeit prüfen | € 797,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-639-8
MDL number:
Specific activity:
≥150 units/mg solid
Biological source:
Streptomyces sp.
€ 276,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenbiological source
Streptomyces sp.
Quality Segment
type
Type VII
form
lyophilized powder
specific activity
≥150 units/mg solid
storage temp.
−20°C
Application
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions. It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.
Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
Biochem/physiol Actions
Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Analysis Note
Protein by biuret
Other Notes
One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.
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Dieser Artikel | |||
|---|---|---|---|
| description Type VII, lyophilized powder, ≥150 units/mg solid | description lyophilized powder, 100-250 units/mg protein | description Type IV, lyophilized powder, ≥100 units/mg solid | description ≥50,000 units/mL, buffered aqueous glycerol solution |
| specific activity ≥150 units/mg solid | specific activity 100-250 units/mg protein | specific activity ≥100 units/mg solid | specific activity ≥50,000 units/mL |
| biological source Streptomyces sp. | biological source - | biological source - | biological source - |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form buffered aqueous glycerol solution |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| type Type VII | type - | type Type IV | type - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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