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Merck

MAK333

Sigma-Aldrich

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(e):

Citric Acid Test Kit

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Aufnahme

culture(s)
plasma
tissue
serum
urine

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

Citrate is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Anwendung

The Citrate Assay Kit may be used for:
  • Cancer Research
  • Drug Discovery
  • Metabolic Disorders Research

Leistungsmerkmale und Vorteile

Efficient and Precise: Detect Citrate levels ranging from 4 to 400 μM (colorimetric) and 0.5 to 40 μM (fluorimetric) in a 15 minute room temperature reaction, providing both convenience and sensitivity.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Eignung

Suitable for citrate determination in biological samples (e.g. plasma, serum, urine, tissue and culture media.)

Prinzip

In this kit, citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.

Sonstige Hinweise

For additional information on our range of Biochemicals, please complete this form.

Lagerklassenschlüssel

10 - Combustible liquids


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