The kit's Technical Bulletin notes that it contains Pyruvate Oxidase, which it utilizes along with Hydrogen Peroxide to create a colored/fluorescent reaction product. Further details characterizing the formulations of the reagents in the kit and the kit's reaction mechanism are proprietary.
Fortfahren mit
MAK332
Pyruvate Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(e):
Pyruvic Acid Detection Kit
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
1 kit | Warenkorb auf Verfügbarkeit prüfen | € 424,00 |
Über diesen Artikel
NACRES:
NA.84
UNSPSC Code:
12161503
Technischer Dienst
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Unterstützung erhaltenusage
sufficient for 100 colorimetric or fluorometric tests
input
biological sample(s)
application(s)
pharmaceutical
detection method
colorimetric, fluorometric
relevant disease(s)
gastrointestinal diseases; cancer
storage temp.
−20°C
General description
Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery.
Application
Suitable for pyruvate detection in biological samples and for studying the effects of drugs on pyruvate metabolism.
The Pyruvate Assay Kit may be used for:
- Drug Discovery
- Food and Beverage Testing
- Neurological Disease Research
Biochem/physiol Actions
The Pyruvate Assay Kit uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λem/ex = 585/530nm is directly proportional to pyruvate concentration in the sample. Linear detection range in 96-well plate: 2 to 500 μM (17 μg/dL to 4.4 mg/dL) pyruvate for colorimetric assays and 0.2 to 50 μM for fluorometric assays.
Features and Benefits
Broad Detection Range: Detect Pyruvate levels accurately across a wide range, from 2 µM to 500 µM, using only 10 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a 30 minute room temperature reaction, reducing complexity and saving valuable time and effort.
Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
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Dieser Artikel | |||
|---|---|---|---|
| description sufficient for 100 colorimetric or fluorometric tests | description sufficient for 100 colorimetric tests | description sufficient for 250 colorimetric tests | description Sufficient for 10 tests |
| detection method colorimetric, fluorometric | detection method colorimetric | detection method colorimetric | detection method colorimetric |
| usage sufficient for 100 colorimetric or fluorometric tests | usage sufficient for 100 colorimetric tests | usage sufficient for 250 colorimetric tests | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| input biological sample(s) | input plasma | input - | input serum |
| relevant disease(s) gastrointestinal diseases; cancer | relevant disease(s) neurological disorders | relevant disease(s) orthopedic diseases; aging/geriatric diseases; hematological disorder; gastrointestinal diseases; neurological disorders; dermatological diseases | relevant disease(s) - |
| application(s) pharmaceutical | application(s) cosmetics | application(s) - | application(s) food and beverages |
Lagerklasse
10 - Combustible liquids
Flammpunkt (°F)
188.6 °F
Flammpunkt (°C)
87 °C
Was ist los?
WGK 2
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Is any of the reagent of this product includes phosphate? I wonder which mechanism or chemical reaction is used for this assay kit (e.g. pyruvate oxidation reaction).
1 answer-
Helpful?
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Could I get a reasonable measurement if I used a 600nm plate reader to measure the output?
1 answer-
A 600 nm excitation wavelength has not been tested for this kit. It is suggested to use 530 nm as the excitation wavelength to achieve reliable results.
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I want to detect E.coli intracellular pyruvate concentration. Therefore, I need to break cells before I detect it. How can I use this kit? I try to use methanol to deal with cells and use freeze-thaw cycle to break. Will methanol have effect to the kit？
1 answer-
This kit has not been tested specifically for use in measuring intracellular pyruvate in E. coli. However, other researchers have used this kit successfully. Methanol is not an interfering substance. Pelleted cells may be quenched and subsequently flash frozen in methanol to lyse. Repeated flash freezing may be applied to assure complete lysis. The combined collected supernatant may then be analyzed by following the recommended protocol. Please see the link below to review.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/358/918/mak332bul.pdfHelpful?
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Can I use the samples in Lactate Assay buffer (Lactate assay kit sigma MAK064) for this kit or does this buffer interfere with the reaction ?
1 answer-
The assay buffer for Kit MAK064 has not been tested to determine suitability with the Kit MAK332.
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