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Merck

MAK105

Sigma-Aldrich

Phosphoglucomutase Colorimetric Assay Kit

sufficient for 100 colorimetric tests

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Lagertemp.

−20°C

Allgemeine Beschreibung

Phosphoglucomutase (PGM) is an enzyme crucial for the interconversion of glucose-1-phosphate and glucose-6-phosphate. In glycogenolysis, PGM converts glucose-1-phosphate into glucose-6-phosphate for use in either the glycolytic pathway or pentose phosphate pathway. When blood glucose levels are high, PGM will convert excess glucose-6-phosphate (formed from glucose by the action of hexokinase) to glucose-1-phosphate.
Deficiency of phosphoglucomutase leads to glycogen storage disease.

Eignung

Suitable for the measurement of phosphoglucomutase activity, analysis of glucose metabolism and cell signaling and screening anti-diabetic drugs in animal tissues (muscle, liver, heart, kidney etc.), cell culture (adherent or suspension cells) and plasma.

Prinzip

The Phosphoglucomutase Colorimetric Assay kit provides a simple and direct procedure for measuring PGM activity in a variety of samples. PGM activity is determined by a coupled enzyme assay in which glucose-1-phosphate is converted by PGM to glucose-6-phosphate. Glucose-6-phosphate is subsequently oxidized to form a product, which reacts with a probe generating a colorimetric (450 nm) product proportional to the PGM activity present. One unit of PGM is the amount of enzyme that will generate 1.0 μmole of NADH per minute at pH 8.0 at room temperature.

Piktogramme

Health hazard

Signalwort

Danger

H-Sätze

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids


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Die Dokumentenbibliothek aufrufen

Liver glucose metabolism in humans.
Adeva-Andany M M, et al.
Bioscience Reports, BSR20160385-BSR20160385 (2016)
Phosphoglucomutase (PGM 1) deficiency: a novel defect of muscle glycogen degradation and synthesis (P07. 200).
Haller R, et al.
Neurology, 78(1), P07-200 (2012)
Feher J J
Quantitative Human Physiology, 1008-1008 (2017)
The enzyme phosphoglucomutase (Pgm) is required by Salmonella enterica serovar Typhimurium for O-antigen production, resistance to antimicrobial peptides and in vivo fitness.
Paterson G K< et al.
Microbiology, 155(10), 3403-3410 (2009)
Jessica N R Peoples et al.
The Journal of biological chemistry, 293(18), 6925-6941 (2018-03-16)
Cardiac energy demands during early embryonic periods are sufficiently met through glycolysis, but as development proceeds, the oxidative phosphorylation in mitochondria becomes increasingly vital. Adrenergic hormones are known to stimulate metabolism in adult mammals and are essential for embryonic development

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