SAE0022

Phosphoglucomutase 1 human

lyophilized powder, recombinant, expressed in E. coli

• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 5.4.2.2 (BRENDA, IUBMB)
• Specific activity: ≥400 units/mg protein
• Assay: ≥95% (PAGE)
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• description: PGM1 isoform sequence with a predicted molecular mass of 61.3kDa.
• assay: ≥95% (PAGE)
• form: lyophilized powder
• specific activity: ≥400 units/mg protein
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Phosphoglucomutase-1 (PGM1) is an evolutionarily conserved enzyme[1] that belongs to the phosphohexose mutase family.[2] It is the major isoform of PGM in skeletal muscle and most other tissues.[3] The PGM1 gene is mapped to human chromosome 1p31.3.[4]

Biochem/physiol Actions

Phosphoglucomutase-1 (PGM1) catalyzes the bidirectional interconversion of glucose-1-phosphate (G-1-P) and glucose-6-phosphate (G-6-P).[1] It regulates carbohydrate metabolism, energy production, and protein N-glycosylation.[2] PGM1 participates in the biosynthesis of nucleotide sugars required for glycan biosynthesis.[5] Variation in the PGM1 gene leads to PGM1 deficiency, which is considered an inherited metabolic disorder in humans.[2][5] PGM1 deficiency causes autosomal recessive diseases such as glycogen storage disease type XIV and congenital disorder of protein N-glycosylation.[5] Affected patients show multiple disease phenotypes, reflecting the central role of the enzyme in glucose homeostasis. The influence of PGM1 deficiency on protein glycosylation patterns is also widespread.[1][6] PGM1 acts as a metabolic tumor suppressor.[4]

Physical form

Supplied as a lyophilized powder containing tris-buffered saline, EDTA, DTT and stabilizer.

Other Notes

One unit will convert 1.0 μmole of α-D-Glucose-1-Phosphate to α-D-Glucose-6-phosphate per minute at pH 7.4 at 30 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable