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MAK055

Sigma-Aldrich

AST-Aktivitäts-Assay-Kit

sufficient for 100 colorimetric tests

Synonym(e):

Aspartat-Aminotransferase-Aktivitäts-Assay-Kit, Glutamat-Oxalacetat-Transaminase-Aktivitäts-Assay-Kit, SGOT-Aktivitäts-Assay-Kit, Serum-Glutamat-Oxalacetat-Transaminase-Aktivitäts-Assay-Kit

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1 KIT
€ 497,00

€ 497,00

Voraussichtliches Versanddatum07. April 2025

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1 KIT
€ 497,00

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

€ 497,00

Voraussichtliches Versanddatum07. April 2025

Bulk-Bestellung anfordern

Verwendung

sufficient for 100 colorimetric tests

Nachweisverfahren

colorimetric

Relevante Krankheit(en)

gastrointestinal diseases; cardiovascular diseases

Lagertemp.

−20°C

Angaben zum Gen

human ... GOT1(2805)
mouse ... GOT1(14718)
rat ... GOT1(24401)

Allgemeine Beschreibung

Das neue AST-Testkit, MAK467, ist jetzt erhältlich! Aspartat-Aminotransferase (AST), ehemals Serum-Glutamat-Oxalacetat-Transaminase (SGOT) genannt, ist ein Pyridoxalphosphat (PLP)-abhängiges Enzym, das die Umwandlung von Aspartat und α-Ketoglutarat in Oxalacetat und Glutamat katalysiert. Wie bei Alanin-Aminotransferase (ALT) werden AST-Werte im Blut gewöhnlich als Leberfunktionsmarker verwendet. AST hat jedoch eine breitere Gewebeverteilung als ALT und die AST-Werte können aufgrund von Erkrankungen oder Verletzungen unterschiedlicher Gewebe (einschließlich Skelett und Herz) schwanken.

Anwendung

Das AST-Aktivitäts-Assay-Kit wird für die Bestimmung der Aktivität von Aspartat-Aminotransferase in Proben verwendet.[1][2]

Leistungsmerkmale und Vorteile

Kompatibel mit Handhabungssystemen mit hohem Durchsatz.

Eignung

Geeignet für die Messung der Aspartat-Aminotransferase (AST)-Aktivität in biologischen Proben, wie Gewebe, Zellen und Serum.

Prinzip

Das AST-Aktivitäts-Assay-Kit ermöglicht ein einfaches, direktes Verfahren für die Messung der AST-Aktivität in verschiedenen Proben. In diesem Kit führt der Transfer einer Aminogruppe von Aspartat zu α-Ketoglutarat zu der Entstehung von Glutamat, was wiederum proportional zu der vorliegenden AST-enzymatischen Aktivität zur Entstehung eines kolorimetrischen Produkts führt (450 nm). Eine AST-Einheit ist definiert als die Menge an Enzym, die 1,0 μmol Glutamat pro Minute bei pH 8,0 und 37 °C generiert.

Ersetzt durch

Produkt-Nr.
Beschreibung
Preisangaben

Piktogramme

Health hazardCorrosion
GHS08,GHS05

Signalwort

Danger

H-Sätze

H314,H334

Gefahreneinstufungen

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B

Lagerklassenschlüssel

8A - Combustible corrosive hazardous materials

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
8A06K07530
8M08K07530
8D13K07530
8G15K07530
8H22K07530

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Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is it possible to use the MAK055 for an experiment that involves evaluating samples with and without B6 added? Additionally, will hemoglobin interfere if a blood hemolysate sample is used when assayed with this kit?

    1 answer

    1. The specific experiment in question has not been performed in the lab, and there are no references for the Aspartate Aminotransferase Activity Assay Kit where a similar experiment has been reported. Considering the nature of the assay and the fact that B6 is a co-enzyme of the ASP, necessary for its activity, in principle, the kit will be suitable to evaluate samples treated with B6. It will be necessary to evaluate any possible interferences and optimize user-specific conditions.
      It is possible that hemoglobin will interfere with the assay if hemolyzed blood is tested. Blood is generally not recommended as a sample, unless specifically mentioned in the assay, due to the possible interference as mentioned. Furthermore, the kit has not been tested with blood samples.

      Helpful?

  4. Does the AST Activity Assay Kit (MAK055), which includes a positive control (MAK055F), have a specific species origin? For instance, is it Human AST?

    1 answer

    1. The positive control in MAK055 is derived from porcine heart.

      Helpful?

  5. Do you anticipate phenol red in culture medium having an impact on the colorimetric read out at 450 nm? (I'm thinking there may be some spectral overlap)

    1 answer

    1. Ideally, culture media should be phenol red-free.

      Helpful?

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