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MAK054

Sigma-Aldrich

α-Ketoglutarate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Nachweisverfahren

colorimetric
fluorometric

Relevante Krankheit(en)

cancer

Lagertemp.

−20°C

Allgemeine Beschreibung

α-Ketoglutarate (α-KG) is a key intermediate in the Tricarboxylic Acid (TCA) Cycle, formed by the oxidative decarboylation of isocitrate by isocitrate dehydrogenase. The synthesis of α-KG from glutamate by glutamate dehydrogenase is a key TCA cycle anaplerotic reaction. α-KG is an important nitrogen transporter. This α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples.

Anwendung

α-Ketoglutarate Assay Kit has been used to determine the activity of α-ketoglutarate.

Eignung

Suitable for the measurement of a-Ketoglutarate in a variety of biological samples including cell and tissue lysates

Prinzip

α-KG concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the α-KG present. Typical sensitivities of detection for this kit are 0.01 to 10 nmoles.

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Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H317,H334

Gefahreneinstufungen

Resp. Sens. 1 - Skin Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

188.6 °F - closed cup

Flammpunkt (°C)

87 °C - closed cup

Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

Phoebe J Benson et al.
Frontiers in microbiology, 7, 475-475 (2016-04-20)
Interest in the production of carbon commodities from photosynthetically fixed CO2 has focused attention on cyanobacteria as a target for metabolic engineering and pathway investigation. We investigated the redirection of carbon flux in the model cyanobacterial species, Synechococcus elongatus PCC
Gunars Osis et al.
Physiological reports, 4(8) (2016-04-28)
The bicarbonate transporter, NBCe1 (SLC4A4), is necessary for at least two components of the proximal tubule contribution to acid-base homeostasis, filtered bicarbonate reabsorption, and ammonia metabolism. This study's purpose was to determine NBCe1's role in a third component of acid-base
Qun Zhou et al.
Bioscience reports, 39(4) (2019-03-25)
Long intergenic non-coding RNA p21 (lincRNA-p21) is down-regulated in some solid tumors. Glutamine catabolism plays an important role in cancer development. However, the role of lincRNA-p21 and its association with glutamine catabolism remain unknown in bladder cancer (BC). In the
Glutamine transporters are targets of multiple oncogenic signaling pathways in prostate cancer
White M A, et al.
Molecular Cancer Research, 480-480 (2017)
Shota Kawakami et al.
Oncology letters, 20(6), 351-351 (2020-10-31)
Isocitrate dehydrogenase 1 (IDH1) mutations are common in gliomas, acute myeloid leukemia, and chondrosarcoma. The mutation 'hotspot' is a single arginine residue, R132. The R132H mutant of IDH1 produces the 2-hydroxyglutarate (2-HG) carcinogen from α-ketoglutarate (α-KG). The reduction of α-KG
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Artikel

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Protokolle

Chiral LC-MS/MS of D- and L-2-Hydroxyglutaric Acid Biomarkers

We describe here a rapid and sensitive method to separate and measure D-2-OHG and L-2-OHG enantiomers using high-resolution mass spectrometry (HRMS) detection.

Unser Team von Wissenschaftlern verfügt über Erfahrung in allen Forschungsbereichen einschließlich Life Science, Materialwissenschaften, chemischer Synthese, Chromatographie, Analytik und vielen mehr..

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