M3769

Medium 199

Name: Medium 199
Brand: SIGMA

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

Synonym(e):

M199 Medium, TCM 199

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NACRES:

NA.71

UNSPSC Code:

12352207

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Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no
phenol red: no
HEPES: no
sodium pyruvate: no
Earle’s salts (5% CO₂): yes
NaHCO₃: no

shipped in

ambient

storage temp.

2-8°C

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Dieser Artikel	M2520	M2154	M0393
Sigma-Aldrich M3769 Medium 199	Sigma-Aldrich M2520 Medium 199	Sigma-Aldrich M2154 Medium 199	Sigma-Aldrich M0393 Medium 199
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
form powder	form powder	form liquid	form powder
components L-glutamine: no phenol red: no HEPES: no sodium pyruvate: no Earle’s salts (5% CO₂): yes NaHCO₃: no	components L-glutamine: yes phenol red: yes Earle’s salts (5% CO₂): yes NaHCO₃: no sodium pyruvate: no HEPES: 25 mM	components sodium pyruvate: no L-glutamine: no NaHCO₃: yes HEPES: no phenol red: yes Earle’s salts (5% CO₂): yes	components HEPES: no Hanks’ salts (2% CO₂): yes sodium pyruvate: no NaHCO₃: no L-glutamine: yes phenol red: yes
shipped in ambient	shipped in ambient	shipped in ambient	shipped in ambient
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C	storage temp. 2-8°C
Quality Level 500	Quality Level 500	Quality Level 400	Quality Level -

General description

Medium 199 ist eine Kombination aus Vitaminen, Aminosäuren und anderen Faktoren, die ursprünglich als vollständig definierte Medienformulierung zum Kultivieren von Primärexplantaten entwickelt wurde. Wenn dieses Medium richtig ergänzt wird, weist es eine breite Anwendbarkeit auf, insbesondere für nicht transformierte Zellen. Medium 199 wird verbreitet für die Vakzinherstellung, In-vitro-Kultivierung von Primärexplantaten des Pankreas und Linsengewebe eingesetzt.

Application

Medium 199 has been used:

to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

Preparation Note

Formulated to contain 9.4 grams of powder per liter of medium.

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

used together

Produkt-Nr.

Beschreibung

Preisangaben

G7513

L-Glutamine solution, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture

P3532

Phenol Red, powder, BioReagent, suitable for cell culture

F2442

Fetal Bovine Serum, USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma

S2GPU05RE

Millipore^® Stericup^® Quick Release Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, pack of 12 ea

S2GPU11RE

Millipore^® Stericup^® Quick Release Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 1000 mL, pack of 12 ea

SEGPU0538

Stericup^® E-GP Funnel-less Sterile Vacuum Filtration System, pore size 0.22 μm, polyethersulfone membrane, filter capacity 500 mL, 38 mm (1 1/2 in.) thread, pack of 12 ea

Lagerklasse

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

0000437175

0000436546

0000388915

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Artikel

Medium 199 - M199 Formulations

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

Protokolle

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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