When reconstituted and filter sterilized, this medium is expected to have a similar shelf-life as prepared 1X Medium 199 with Hanks salts and L-glutamine. See product 51322C for the prepared liquid medium. This product has an expiration date of 1 year from the date of quality release. Upon the addition of serum or other heat-sensitive components, the shelf life would be limited to 1 - 2 weeks at 2 - 8°C. Freeze/thaw cycles may result in precipitate formation.
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Über diesen Artikel
Produktname
Medium 199, With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
HEPES: no
Hanks’ salts (2% CO2): yes
sodium pyruvate: no
NaHCO3: no
L-glutamine: yes
phenol red: yes
shipped in
ambient
storage temp.
2-8°C
General description
Application
- to test and determine the pH sensitivity of the sensors in a complex environment
- as a culture medium during oocyte harvesting
- as a component of Leishmania donovani (LdBOB) promastigote media for culturing L. donovani
- to culture visceral adipose tissue explants
Preparation Note
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| description With Hanks′ salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture | description HEPES Modification, with Earle′s salts, L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture | description Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture | description With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| form powder | form powder | form powder | form liquid |
| components HEPES: no | components L-glutamine: yes | components L-glutamine: no | components sodium pyruvate: no |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 400 |
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Lagerklasse
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
Zulassungslistungen
Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.
10043-52-4
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Once prepared, how long can the media stay refrigerated? Can it be frozen?
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