This product has not been tested with synthetic substrate pNP-Palmitate in-house. Its activity on pNP-Palmitate has been cited in the following article that may be of interest as a reference: Int J Biol Macromol. 2023 Mar 1;230:123427.
Fortfahren mit
L3126
Lipase aus Schweinepankreas
Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Synonym(e):
Pankreatische Lipase, Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-619-9
EG-Nummer:
MDL number:
Specific activity:
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
Biological source:
Porcine pancreas
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenbiological source
Porcine pancreas
Quality Segment
type
Type II
form
lyophilized (cake)
specific activity
≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin)
composition
protein, ≥20%
storage temp.
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
InChI key
QWZUIMCIEOCSJF-CHHCPSLASA-N
Application
Pankreatin aus der Bauchspeicheldrüse (Pankreas) vom Schwein findet Verwendung:
Lipasen werden in der Industrie zum Auflösen von chiralen Verbindungen und zur Umesterung bei der Herstellung von Biodiesel verwendet.
Biochem/physiol Actions
Lipasen katalysieren die Hydrolyse von Estern in wässrigen Lösungen und die Synthese von Estern in nicht wässrigen Lösungen. Darüber hinaus produzieren sie Hydroxyl- und Carboxylgruppen durch die Hydrolyse von Esterverbindungen in Poly(ethylenterephthalat).
Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.
Lipasen katalysieren die Hydrolyse von Triacylglycerinen zu Glycerin und freien Fettsäuren.
Analysis Note
Proteinbestimmung nach Biuret.
Roh (Steapsin). Enthält Amylase- und Proteaseaktivität.
Other Notes
Eine Einheit hydrolisiert 1,0 mikroäquivalente Fettsäure aus Triacetin in einer Stunde bei einem pH-Wert von 7,4 und einer Temperatur von 37°C (pH-Wert 7,7 bei Olivenölsubstrat)
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Dieser Artikel | |||
|---|---|---|---|
| description Type II, ≥125 units/mg protein (using olive oil (30 min incubation)), 30-90 units/mg protein (using triacetin) | description Type VI-S, ≥20,000 units/mg protein, lyophilized powder | description Type I, lyophilized powder, 5-15 units/mg solid | description lyophilized powder, ≥40,000 units/mg protein |
| biological source Porcine pancreas | biological source - | biological source wheat (germ) | biological source fungus (Candida rugosa) |
| specific activity 30-90 units/mg protein (using triacetin), ≥125 units/mg protein (using olive oil (30 min incubation)) | specific activity ≥20,000 units/mg protein | specific activity 5-15 units/mg solid | specific activity ≥40,000 units/mg protein |
| form lyophilized (cake) | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| composition protein, ≥20% | composition Protein, 40-70% | composition - | composition Protein, ≥4.0% |
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Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
PSA (Persönliche Schutzausrüstung)
Eyeshields, Gloves, type N95 (US)
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
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Can I use the synthetic substrate pNP-Palmitate to perform the activity assay, or does this enzyme only have activity with a natural substrate?
1 answer-
Helpful?
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How was the olive oil prepared for the activity determination? Was it emulsified? If yes, in which oil:water ratio? Was any emulsifier used?
1 answer-
This product contains 50% (v/v) olive oil (catalog number O1500).
1% sodium azide is added as a preservative and an emulsifier. The exact composition and method of
composition is considered proprietary and therefore will not be disclosed.Helpful?
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May I know the PDB information related to this lipase protein?
1 answer-
PDB information is not available for this product.
Helpful?
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I want to use this product (L3126) for preparing a solution to conduct in vitro degradation experiments on a particular material. Could you please provide detailed instructions on how to prepare the solution, including any recommendation?
1 answer-
Unfortunately, there is no application specific instructions available for this product. The material may be reconstituted in 100 mM potassium phosphate buffer, pH 8.0, containing 1 mg/ml BSA. This product is from porcine pancreas. Another product option, L3419 Lipase from Thermus thermophilus, is reconstituted in 50 mM Tris-HCl, pH 8.0. This material states that solutions are stable for up to 1 month at 2 - 8°C. Please see the link below to review the datasheet for this item:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/504/l3419dat.pdfHelpful?
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How is a "microequivalent" of fatty acid quantified in the enzymatic activity unit definition for L3126?
1 answer-
A microequivalent is equivalent to 1 mmol/L. In this case, one unit of Lipase will hydrolyze 1.0 microequivalent (1 mmol/L) of fatty acid from olive oil in 1 hour at pH 7.7 at 37°C.
Helpful?
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