L3001

Lipase aus Weizenkeimen

Type I, lyophilized powder, 5-15 units/mg solid

• Synonym(e): Triacylglycerol-acylhydrolase, Triacylglycerol-lipase
• CAS-Nummer: 9001-62-1
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-619-9
• EG-Nummer: 3.1.1.3 (BRENDA, IUBMB)
• MDL number: MFCD00131509
• Specific activity: 5-15 units/mg solid
• Biological source: wheat (germ)

Eigenschaften

• biological source: wheat (germ)
• Quality Segment: 200
• type: Type I
• form: lyophilized powder
• specific activity: 5-15 units/mg solid
• UniProt accession no.: Q8L6B0
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N
• Gene Information: wheat ... lipase(543006)

Beschreibung

General description

Lipases belong to the class of hydrolases. Microbes of plant and animal basis mainly produce lipase enzyme. It can also be produced by marine organisms.

Application

Lipase from wheat germ has been used:

• to prepare enzyme solution for the biodegradability test
• to treat diacetates of Mep-Xylp, to generate monoacetylated xylosides
• to test its efficacy in selenium speciation analysis and to determine the contribution that impurities could make to Se determination in real samples

Biochem/physiol Actions

Tri-, Di-, und Monoglyceride sind hydrolysiert (in abnehmender Ratenreihenfolge).

Lipases are used in detergent, food, leather, pharmaceutical and dairy industries. It has several characteristics like substrate specificity, stereospecificity and regioselectivity. It also participates in various bioconversion reactions like, hydrolysis, esterification, acidolysis and aminolysis.

Preparation Note

Prepared essentially by method of Singer, T.P., J. Biol. Chem., 174, 11 (1948).

Analysis Note

Contains acid phosphatase.

Other Notes

One unit will hydrolyze 1.0 microequivalent of fatty acid from a triglyceride in 1 hr at pH 7.4 at 37 °C. (This is equivalent to approx. 10 microliters of CO₂ in 30 minutes.)

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• Gefahrencodes: H334
• Sicherheitshinweise: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• PSA (Persönliche Schutzausrüstung): Eyeshields, Gloves, type N95 (US)