Unfortunately, a Krebs-Ringer Bicarbonate Buffer without glucose is not available.
K4002
Krebs-Ringer Bicarbonate Buffer
With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture
Synonym(e):
Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution
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Über diesen Artikel
NACRES:
NA.75
UNSPSC Code:
12161702
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Unterstützung erhaltenform
powder
technique(s)
cell culture | mammalian: suitable
pH
6.1-6.7
solubility
water: 9.5 g/L, clear
storage temp.
2-8°C
Quality Level
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 500 |
| form powder | form powder | form liquid | form powder |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C |
| solubility water: 9.5 g/L, clear | solubility - | solubility - | solubility - |
| pH 6.1-6.7 | pH - | pH - | pH - |
General description
Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.
Application
Krebs-Ringer Bicarbonate Buffer has been used:
- as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay
- in the pre-perifusion for islet viability and functionality studies[1]
- in pituitary bioassay
Preparation Note
Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 1.26 g/L sodium bicarbonate.
Lagerklasse
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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N C Schaad et al.
Human reproduction (Oxford, England), 11(3), 561-565 (1996-03-01)
Nitric oxide is a chemical messenger which functions as a neurotransmitter or as a cytotoxic agent. Nitric oxide synthase (NOS) has been isolated from various mammalian reproductive tissues. The presence or absence of NOS in spermatozoa has not yet been
Bidisha Dasgupta et al.
European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
Sandra E Pineda-Sanabria et al.
Journal of molecular and cellular cardiology, 92, 174-184 (2016-02-09)
One approach to improve contraction in the failing heart is the administration of calcium (Ca(2+)) sensitizers. Although it is known that levosimendan and other sensitizers bind to troponin C (cTnC), their in vivo mechanism is not fully understood. Based on
Protokolle
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
Verwandter Inhalt
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Do you have a glucose free product?
1 answer-
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
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How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?
1 answer-
Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.
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What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?
1 answer-
The formulation for KRB can be found on the data sheet.
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Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?
1 answer-
Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity. If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM. You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.
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