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K3753

Krebs-Henseleit Buffer Modified

With 2000 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

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UNSPSC Code:
12352207
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Quality Segment

500

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

General description

Krebs-Henseleit Buffer Modified is a balanced salt solution (BSS) that is used in cell culture and plays a role in providing cells with water and inorganic salts for normal cell metabolism. In addition, it provides a buffering system to maintain the optimal pH of the medium and acts as an irrigating, transporting, and diluting agent to maintain the intra- and extra-cellular osmotic balance. BSS provides glucose as an energy source for cell metabolism.

Application

Krebs-Henseleit Buffer Modified has been used:

  • to collect the rat brains for ex vivo brain slice experiments
  • to measure calcium and sodium in rat pulmonary arterial endothelial cells (PAECs)[1]
  • to collect the uterine and forelimb blood vessel samples from horses[2]

Preparation Note

Formulated to contain 9.6 grams of powder per liter of medium.
Supplement with 2.1 g/L sodium bicarbonate.

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Dieser Artikel
K4002T2397H2387
Sigma-Aldrich

Sigma-Aldrich

K3753

Krebs-Henseleit Buffer Modified

Krebs-Ringer Bicarbonate Buffer With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

K4002

Krebs-Ringer Bicarbonate Buffer

Tyrode’s Salts With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

T2397

Tyrode′s Salts

Hanks-Salze Modified, without calcium chloride, magnesium sulfate and sodium bicarbonate, powder, suitable for cell culture

Sigma-Aldrich

H2387

Hanks-Salze

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

Quality Level

500

Quality Level

500

Quality Level

400

Quality Level

-

form

powder

form

powder

form

liquid

form

powder

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

room temp

storage temp.

2-8°C

Lagerklasse

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Questions

  1. How long can Krebs solution be stored after it is being reconstituted?

    1 answer

    1. Stability data for Krebs-Henseleit Buffer Modified (cat# K3753) when reconstituted is not available. Stability will also depend on the supplements added to the solution.

      It is generally accepted that the solution can be stored between 2 and 8°C for several months, on the condition that it has been filtered using a 0.2µm membrane in order to make sterile. If it is not sterile, it must be used within 24 hours.

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