I17003

Interleukin-4 human

IL-4, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

• Synonym(e): Interleukin-4 human, IL-4
• MDL-Nummer: MFCD00151705
• UNSPSC-Code: 12352202
• NACRES: NA.77

Eigenschaften

• Rekombinant: expressed in HEK 293 cells
• Qualitätsniveau: 200
• Assay: ≥98% (SDS-PAGE)
• Wirksamkeit: ≤1 ng/mL ED₅₀
• Mol-Gew.: 14.9 kDa (glycosylated)
• Methode(n): cell culture | mammalian: suitable
• Eignung: endotoxin tested
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Recombinant human Interleukin-4 (IL-4) is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 14.9 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

Biochem./physiol. Wirkung

IL-4 is a protein that has been shown to regulate a wide spectrum of functions in B cells, T cells, monocytes/macrophages and other haematopoietic and non-haematopoietic cells. Among T cell clones, IL-4 is produced by Th0 and Th2 cells, but not Th1 cells, and this has now been demonstrated both in mice and in humans. IL-4 blocks the production of proinflammatory cytokines such IL-6, TNFα, and M-CSF and improves the differentiation of immature DCs from CD34+ progenitors when added during the differentiation period (day 6 to day 12).

Interleukin-4 is a lymphokine with profound effects on the growth and differentiation of immunologically competent cells. IL-4 is also known as B cell stimulatory factor-1 (BSF-1), T cell growth factor-2 (TCGF-2) and mast-cell growth factor-2 (MCGF-2). Inhibits VEGF-induced and bFGF-induced angiogenesis. IL-4 is a complex glycoprotein released by a subset of activated T cells. Human and mouse IL-4 share 50% amino acid sequence homology, but their biological actions are species-specific.

Interleukin-4, a lymphokine with profound effects on the growth and differentiation of immunologically competent cells, inhibits VEGF-induced and bFGF-induced angiogenesis. Human and mouse IL-4 share 50% amino acid sequence identity, but their biological actions are species-specific.

Sequenz

HKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS

Physikalische Form

Supplied as a lyophilized powder containing phosphate buffered saline.

Hinweis zur Analyse

The biological activity of recombinant human IL-4 was tested in culture by measuring its ability to stimulate proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable