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GE28-9574-99

TALON® Superflow™

Cytiva 28-9574-99, pack of 10 mL

Synonym(e):

TALON Affinity Resin

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About This Item

UNSPSC-Code:
23151817
NACRES:
NA.56

ligand

TALON® Superflow Cobalt-IMAC

Verpackung

pack of 10 mL

Hersteller/Markenname

Cytiva 28-9574-99

Matrix

6% cross-linked agarose

Partikelgröße

60-160 μm

cleaning in place

2-14(Co2+-stripped medium)

working range

3-12(Co2+-stripped medium)

Kapazität

≥20 mg binding capacity (histidine-tagged protein)(The binding capacity for individual proteins may vary)

Lagertemp.

2-8°C

Allgemeine Beschreibung

Talon® Superflow is a cobalt-based IMAC medium offering different selectivity compared to nickel-charged media.

Leistungsmerkmale und Vorteile

  • Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant Proteins by immobilized metal affinity chromatography (IMAC).
  • Suitable for IMAC purification of histidine-tagged Proteins when Ni2+ is not the optimal choice of metal ion.
  • Compatible with commonly used IMAC reagents and appropriate for purifying Proteins under native or denaturing conditions.
  • Allows for Protein purification under native or denaturing conditions and can be used with prokaryotic and eukaryotic expression systems.

Lagerung und Haltbarkeit

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Hinweis zur Analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Rechtliche Hinweise

Superflow is a trademark of Sterogene Bioseparations Inc.
Talon is a registered trademark of Clontech

Piktogramme

Flame
GHS02

Signalwort

Warning

H-Sätze

H226

Lagerklassenschlüssel

3 - Flammable liquids

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Artikel

Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns

His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.

Cell Lysis of Histidine-Tagged Proteins

Follow established procedures for recombinant protein expression, growth, induction, and cell lysis optimization.

Optimierung der Aufreinigung von Histidin-markierten Proteinen

Optimierung der Aufreinigung von Histidin-markierten Proteinen mit Cytiva-Produkten.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using Cytiva products.

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Protokolle

Purification of Histidine-Tagged Proteins using TALON® Superflow

This page shows how to purify histidine-tagged proteins using TALON Superflow from Cytiva.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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