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F4135

Sigma-Aldrich

Fetales Kälberserum

Fetal Bovine Serum

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

Synonym(e):

HI-FBS, HI-FCS, HI-Seren, HI-Serum, FBS, FCS, Seren, Serum

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About This Item

MDL-Nummer:
MFCD00132239
UNSPSC-Code:
12352207
eCl@ss:
32160409
NACRES:
NA.71
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Biologische Quelle

bovine fetus

Qualitätsniveau

500

Sterilität

sterile-filtered

Produktlinie

FBS Classic

Zusammensetzung

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

Ursprung

USA origin

Methode(n)

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

Verunreinigungen

≤10 EU/mL endotoxin

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

Fötales Kälberserum (FBS) ist ein gebräuchlicher Medienzusatz, da es zahlreiche Funktionen in der Zellkultur ausübt. FBS liefert Nährstoffe sowie Wachstums- und Anhaftungsfaktoren und unterstützt die Proliferation.[1] Es schützt die Zellen außerdem vor oxidativer Schädigung und Apoptose.[2] FBS verhindert die Bildung von reaktiven Sauerstoffspezies, die in serumfreien Medien (SFM) nicht ausgeschlossen ist.[3]

Anwendung

FBS aus den USA wird für zahlreiche Zellkulturanwendungen eingesetzt. FBS bietet viele undefinierte wachstumsfördernde und überlebensverbessernde Faktoren für Kulturzellen.
Fötales Kälberserum wurde in folgenden Anwendungen eingesetzt:
  • als Bestandteil von Dulbecco′s Modified Eagle′s Medien (DME, DMEM),[4] Eagle′s Minimum Essential Medium (EMEM),[5] Roswell Park Memorial Institute Medium (RPMI-1640),[6] α-Modified Eagle‘s Medium (αMEM) (MEM)[7] und Leibovitz L-15 Medium[8]
  • als Komponente des Blockierungspuffers zur Behandlung von Hirnstammschnittpräparaten[9]
  • zur Verbesserung der Anheftung von Hautexplantaten[10]

Angaben zur Herstellung

Dieses Produkt wird durch Erwärmen bei 56 °C für 30 Minuten hergestellt. Für Immunassays wird das Komplementsystem durch Erwärmen des Serums auf 56 °C für 30 Minuten inaktiviert. Die Wärmeaktivierung inaktiviert auch andere, unbestimmte Inhibitoren des Zellwachstums in Kultur.
Hergestellt durch Erwärmen bei 56 °C für 30 Minuten.

Hinweis zur Analyse

  • Auf Bakterien, Viren und Mykoplasmen getestet
  • Unter aseptischen Bedingungen dreifach durch eine 0,1-μm-Membran filtriert.

  • Endotoxin- und Hämoglobin-getestet

auch häufig zusammen mit diesem Produkt gekauft

Produkt-Nr.
Beschreibung
Preisangaben

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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SAFC

SAFC

55037C

Hanks-Salze -Lösung

Hypoxia enhances secretion of matrix metalloproteinases in bovine dermal fibroblasts: an in vitro approach to bovine digital dermatitis
Akkoc A, et al.
Journal of Applied Animal Research, 46, 445-450 (2018)
Antileishmanial activity study and theoretical calculations for 4-amino-1, 2, 4-triazole derivatives
Suleymanouglu N, et al.
Journal of Molecular Structure, 1144, 80-86 (2017)
Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
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Protect your investment with best practices for storing and thawing FBS and other sera. Sample FBS for validation in your cell culture protocol.

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Questions

1–6 of 6 Questions  

  1. Is it possible to store the FBS at a temperature of -80°C, which is lower than the recommended storage temperature of -20°C?

    1 answer

    1. The FBS should be stored at the recommended temperature of -20°C. Storage at -80°C has not been tested, but there are no anticipated issues. Frost Free Freezers should be avoided for storage.

      Helpful?

  2. Recommended storage is listed as -20C; is it safe/ok to store at -80C?

    1 answer

    1. The recommended long-term storage temperature for FBS is -20C. For the short-term, such as for shipping, packing on dry ice at -40C is recommended. It is not recommended to store the FBS at temperatures lower than -40C. Please see the guide on freezing and thawing FBS and other sera below:
      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-culture-media-preparation/storing-and-thawing-serum

      Helpful?

  3. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer

    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. Why is serum Heat-Inactivated (HI)?

    1 answer

    1. Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.

      Helpful?

  6. Does Sigma-Aldrich offer delipidated bovine serum?

    1 answer

    1. At this time, Sigma-Aldrich does not offer delipidated bovine serum (either FBS or calf serum).A method for lipid depletion, though not necessarily that of Sigma:1. While mixing, gradually add 10 g fumed silica (Product S5130 or Aerosil) per liter of serum2. Mix at room temperature and then transfer to 2-8 °C for overnight mixing.3. After overnight mixing, remove flumed silica (Aerosil) by centrifugation at 3000-5000 rpm for 5 minutes.4. Retain the supernatant. The lipids should stick to the silica. There may be some protein loss

      Helpful?

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