GH1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC-Code: 41106514

Eigenschaften

• Biologische Quelle: rat brain
• Verpackung: tube of 5 μg 89072610-DNA-5UG; pkg of vial of cells 89072610-1VL
• Wachstumsmodus: Semi-adherent
• Karyotyp: Hypodiploid, modal no. 40.
• Morphologie: Epithelial
• Produkte: Rat growth hormone, lactogenic hormones.
• Rezeptoren: Not specified
• Methode(n): cell culture | mammalian: suitable
• Relevante Krankheit(en): cancer
• Versandbedingung: dry ice
• Lagertemp.: −196°C

Beschreibung

Ursprung der Zelllinie

Rat Wistar-Furth pituitary tumour

Beschreibung der Zelllinie

Derived from pituitary gland of Wistar-Furth rats. The cells secrete growth hormone at a rate of 1μg/ml cell protein/day. Re-inoculation of cloned cells back to Wistar-Furth rats results in a marked increase in body weight and stimulation of linear growth in both intact and hypophysectomized animals independent of rat tumour size.

Anwendung

Hormone studies

Nährmedium

Ham′s F10 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subkultur-Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. These cells only semi-attach and may return to suspension during transit. If this has occurred on receipt of the cells pellet cells b

Sonstige Hinweise

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