Near tetraploid; range 88 to 91 - see Cell Line Description for more information
Morphologie
Fusiform, can form heaps of cells
Produkte
Not specified
Rezeptoren
Not specified
Methode(n)
cell culture | mammalian: suitable
Relevante Krankheit(en)
cancer
Versandbedingung
dry ice
Lagertemp.
−196°C
Ursprung der Zelllinie
Human glioblastoma
Beschreibung der Zelllinie
A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2;37ºC.
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