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PKH26GL

PKH26 Linker-Kit für rot fluoreszierende Zellen für die allgemeine Zellmembranmarkierung

Distributed for Phanos Technologies

Synonym(e):

Red PKH membrane dye

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Ihnen/SKUVerfügbarkeitPreis
1 kit
Warenkorb auf Verfügbarkeit prüfen
€ 1.750,00

Über diesen Artikel

NACRES:
NA.32
UNSPSC Code:
12352207

€ 1.750,00

Warenkorb auf Verfügbarkeit prüfen
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Technischer Dienst
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Unterstützung erhalten

packaging

pkg of 1 kit

Quality Segment

200

manufacturer/tradename

Distributed for Phanos Technologies

storage condition

protect from light

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

detection method

fluorometric

shipped in

ambient

storage temp.

room temp

General description

PKH26 ist ein rot fluoreszierender Farbstoff. Dieser lipophile Membranfarbstoff wird vielfach eingesetzt, um die Zellproliferation in heterogenen Zellen mittels Durchflusszytometrie zu bestimmen.

Application

Dieses Kit ist für die allgemeine Zellmembranmarkierung vorgesehen. Es wurde in einer Reihe an Modellsystemen charakterisiert und erwies sich als nützlich bei der In-vitro-Zellmarkierung, bei In-vitro-Proliferationsstudien und bei langzeitiger In-vivo-Beobachtung. Die Halbwertszeit für die Elution von PKH26 aus markierten Erythrozyten von Kaninchen beträgt mehr als 100 Tage. Diese verbesserte Stabilität ist vorteilhaft für In-vivo-Langzeitstudien.
Das PKH26-Linker-Kit für rot fluoreszierende Zellen ist für die allgemeine Zellmembranmarkierung vorgesehen. Es wurde in einer Reihe an Modellsystemen charakterisiert und erwies sich als nützlich bei der In-vitro-Zellmarkierung, bei In-vitro-Proliferationsstudien und bei langzeitiger In-vivo-Beobachtung.[1] Die Halbwertszeit für die Elution von PKH26 aus markierten Erythrozyten von Kaninchen beträgt mehr als 100 Tage. Diese verbesserte Stabilität ist vorteilhaft für In-vivo-Langzeitstudien.

Other Notes

Für weitere technische Details zu PKH und fluoreszierenden CellVue®-Zell-Linker-Farbstoffen, einschließlich einer umfangreichen Bibliografie, klicken Sie bitte hier.

Legal Information

CellVue is a registered trademark of Phanos Technologies

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Dieser Artikel
PKH26PCLPKH67GLP7458
PKH26 Linker-Kit für rot fluoreszierende Zellen für die allgemeine Zellmembranmarkierung Distributed for Phanos Technologies

Sigma-Aldrich

PKH26GL

PKH26 Linker-Kit für rot fluoreszierende Zellen für die allgemeine Zellmembranmarkierung

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Distributed for Phanos Technologies

Sigma-Aldrich

PKH26PCL

PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling

PKH67 grün fluoreszierendes Zelllinker-Kit für die allgemeine Zellmembranmarkierung Distributed for Phanos Technologies

Sigma-Aldrich

PKH67GL

PKH67 grün fluoreszierendes Zelllinker-Kit für die allgemeine Zellmembranmarkierung

PKH26 Reference Microbeads

Sigma-Aldrich

P7458

PKH26 Reference Microbeads

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

2-8°C

fluorescence

λex 551 nm; λem 567 nm (PKH26 dye)

fluorescence

-

fluorescence

λex 490 nm; λem 502 nm (PKH67 dye)

fluorescence

-

packaging

pkg of 1 kit

packaging

-

packaging

pkg of 1 kit

packaging

-

manufacturer/tradename

Distributed for Phanos Technologies

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

detection method

fluorometric

detection method

-

detection method

fluorometric

detection method

fluorometric

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Diluent C 6 x 10
  • PKH26 Cell Linker in ethanol .5 mL

pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

hcodes

H225,H319

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Lagerklasse

3 - Flammable liquids

wgk

WGK 1

flash_point_f

57.2 °F - closed cup

flash_point_c

14.0 °C - closed cup

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Lot/Batch Number

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Questions

1–10 of 11 Questions  

  1. Can we stain extracellular vesicles particularly exosomes (isolated and suspended in PBS) we have used exosome isolation kit , is ultracentrifugation step must at the end, as for now we are not using ultracentifuge

    1 answer

    1. PKH dyes have been successfully used to label exosomes and extracellular vesicles for in vitro and in vivo tracking experiments. The exosome labeling protocol below details steps for reliable fluorescent labeling of exosomes using PKH dyes for micro vesicle tracking experiments. Instead of PBS, use the kit’s recommended Diluent C for optimal dye performance. Final resuspension in PBS is acceptable for downstream applications.

      Please check this link for more details:
      https://www.sigmaaldrich.com/technical-documents/protocols/biology/cell-culture/exosome-labeling-pkh.html

      These links could also be helpful to you:
      https://www.sigmaaldrich.com/catalog/product/sigma/mini26?lang=en&region=US
      https://www.sigmaaldrich.com/technical-documents/articles/biowire/cell-tracking-lipophilic-membrane-dyes.html

      Helpful?

  2. If we want to use the PKH to label the membrane of fibroblast, and then co-culture them with tumor cells in order to interact, ¿ Will PKH transfer from fibroblast to tumor cells?

    1 answer

    1. PKH dyes are routinely used in co-culture situations. Because staining is nearly instantaneous, rapid and homogeneous dispersion of cells in dye solution is essential. There should be no excess or unincorporated dye in the medium prior to mixing or co-culturing the cell lines. Free dye will be taken up by all cells. See the links below to the product protocol as well as two additional co-culture studies using PKH dyes:

      Protocol
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/271/475/mini26bul.pdf

      Co-culture in scaffolds
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5155425/

      Co-culture, mixed monolayer, and on Transwell
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5168417/

      Helpful?

  3. Are cells lost during the PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling staining process?

    1 answer

    1. Over-labeling of the cells will result in loss of membrane integrity and reduced cell recovery.  Methods for improving cell viability can be found on the troubleshooting guide.

      Helpful?

  4. Product PKH26GL, PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, is a red dye.  Do you offer a kit that stains the cells green?

    1 answer

    1. We offer Product numbers:PKH2GL Both of these kits stain the cells with a dye that emits in the green fluorescent range.

      Helpful?

  5. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  6. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

  7. Does the dye leak from the cells after labeling when using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling?

    1 answer

    1. In general, slow loss of fluorescence has been observed with PKH1 and PKH2 in in vivo studies and it is likely that PKH67 may also slowly lose fluorescence. Leakage (or loss of staining intensity) may also occur due to cell-to-cell transfer of the dye. The most common cause of cell-to-cell dye transfer is inadequate washing of the cells. Wash cells 3-5 times after labeling and transfer samples to new tubes between washes.

      Helpful?

  8. What is the difference between PKH26 Red Fluorescent Cell Linker Kit for Phagocytic Cell Labeling Products PKH26GL and PKH26PCL?

    1 answer

    1. The general labeling kit (Product No. PKH26GL) will label the membrane of the cells.  For phagocytic cells, Product No. PKH26PCL is used.  This kit utilizes dye aggregates for specific labeling of phagocytic cells in the presence of other cell types.

      Helpful?

  9. How many cells can be stained with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling, Products PKH26GL and MINI26?

    1 answer

    1. For Product PKH26GL, when using the concentrations indicated in the product bulletin, you can stain 30 samples (1x107 cells/sample).The kit provides 500 ul of PKH26 dye solution.  You use 4 ul per sample.  There is enough dye in the kit to stain 125 samples.The kit provides 60 mls of Diluent C.  You use 2 mls per sample (1 ml for dilution of the dye, 1 ml for dilution of the cells).  There is enough diluent for 30 samples.You can purchase additional diluent as Product CGLDILIf you need less dye, you can purchase Product MINI26 which contains 100 ul of dye and 10 mls of Diluent C.  This kit contains enough dye to stain 25 samples and enough diluent to stain 5 samples.

      Helpful?

  10. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

1–10 of 11 Questions  

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