AGS Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 89090402, human stomach, Not specified

• UNSPSC Code: 41106514
• Biological source: human stomach
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Hyperdiploid
• Morphology: Not specified
• Manufacturer/tradename: ECACC

Eigenschaften

• biological source: human stomach
• description: Human Caucasian gastric adenocarcinoma
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89090402-DNA-5UG, pkg of vial of cells 89090402-1VL
• growth mode: Adherent
• karyotype: Hyperdiploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

AGS human cell line has been used:

• to study the role of obestatin/G protein-coupled receptor 39 (GPR39) in gastric cancer progression
• to determine expression levels of colon cancer associated transcript -1 (CCAT1)
• to study the adhesion of Lactobacillus sps. to the gastric mucosa

Anti-tumour testing

Biochem/physiol Actions

Derived from an adenocarcinoma of the stomach of a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Human Caucasian gastric adenocarcinoma

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 11,12
vWA: 16,17

Preparation Note

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 0.05% EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.