Here is the link to our Biochemical Solubility table:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/287/917/c3045inf.pdf
For the Cell Culture Tested grade of this product, C3045, three options are provided: Serum, Tween and Ethanol.
Note that if Serum is used as the solvent, it may already contain some Cholesterol. The "Tween" is referring to an aqueous solution of either TWEEN 20 or TWEEN 80. Also, Ethanol can be used to make a stock solution, before supplementing the culture medium; consider perhaps a 100X solution in ethanol, which would give a concentration of 1 percent ethanol in the medium.
C8667
Cholesterin
Sigma Grade, ≥99%
Cholesterol
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
3β-Hydroxy-5-cholesten, 5-Cholesten-3β-ol
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500 MG
€ 38,70
1 G
€ 49,00
5 G
€ 84,30
25 G
€ 279,00
100 G
€ 891,00
Über diesen Artikel
Empirische Formel (Hill-System):
C27H46O
CAS-Nummer:
Molekulargewicht:
386.65
NACRES:
NA.77
PubChem Substance ID:
eCl@ss:
39023139
UNSPSC Code:
12352111
EC Number:
200-353-2
MDL number:
Beilstein/REAXYS Number:
1915888
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Unterstützung erhaltenbiological source
sheep wool
Quality Level
grade
Sigma Grade
assay
≥99%
form
powder
bp
360 °C (lit.)
mp
147-149 °C (lit.)
solubility
water: soluble 0.00003 g/L at 20 °C
density
1.067 g/mL at 25 °C (lit.)
shipped in
ambient
storage temp.
−20°C
SMILES string
CC(C)CCC[C@@H](C)[C@H]1CC[C@H]2[C@@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C
InChI
1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3/t19-,21+,22+,23-,24+,25+,26+,27-/m1/s1
InChI key
HVYWMOMLDIMFJA-DPAQBDIFSA-N
Gene Information
human ... POLA1(5422), TOP2A(7153)
rat ... Ar(24208), Polb(29240)
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Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| assay ≥99% | assay ≥99.0% (GC) | assay ≥90% (HPLC) | assay >99% (TLC) |
| biological source sheep wool | biological source lanolin | biological source - | biological source - |
| grade Sigma Grade | grade - | grade - | grade - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 10-30°C | storage temp. −20°C |
| form powder | form powder | form powder | form powder |
| shipped in ambient | shipped in - | shipped in ambient | shipped in dry ice |
Application
Standard für die Chromatographie
Cholesterin wurde für die Präparation von immunstimulierenden Komplexen verwendet, um die zellulären und humoralen Immunantworten zu verstärken.[1] Es wurde Tierfutter beigemischt, um die Auswirkungen einer cholesterinreichen Ernährungsweise zu untersuchen.
Biochem/physiol Actions
Cholesterin ist ein Lipid, das zwischen 20 und 25 % der Strukturkomponenten in Zellmembranen ausmacht. Es bestimmt die Fluidität und Durchlässigkeit der Membran und macht sie für Wasser durchlässig, nicht aber für Ionen und Protonen. Cholesterin reguliert die Transportfunktionen und signalisiert das Vorhandensein von Proteinen auf der Plasmamembran.[2] Die Cholesterinsynthese erfolgt hauptsächlich im Dünndarm und in der Leber.[3]
Hauptbestandteil biologischer Membranen; ca. 25% des gesamten Lipids im Gehirn ist Cholesterin.
Preparation Note
Cholesterin-Monohydrat bildet in heißem Alkohol eine klare, farblose bis leicht gelbliche Lösung. Es ist auch in Aceton, Dioxan, Ethylacetat, Benzol, Petrolether, Ölen, Fetten und in wässrigen Gallensalzlösungen löslich. Die Lösungen sollten vor Licht geschützt aufbewahrt werden.
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Artikel
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| C8667-100G | 04061835543601 |
| C8667-5G | 04061835543632 |
| C8667-1G | 04061835543618 |
| C8667-25G | 04061835521746 |
| C8667-500MG | 04061835543625 |
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how to prepare for cell culture? thank you
1 answer-
Helpful?
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What is the best temperature for storage for 5 mg/mL solutions in ethanol? Can they be stored at 4C? Thank you!
1 answer-
The stability of this material in solution at 2-8°C has not been evaluated. Solutions prepared in Chloroform, in aliquots and protected from light, are stable for at least 1 year when stored at -20°C. Solutions prepared in Ethanol are expected to be similarly stable.
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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Buongiorno, volevo chiedere cortesemente quale sia l'origine del prodotto
1 answer-
This product is derived from sheep wool. The origin information of this product will be listed on the lot-specific Certificate of Origin. Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a Certificate under the ‘Certificate of Origin’ tab:
https://www.sigmaaldrich.com/product/sigma/c8667#product-documentationHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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Hi, I want to know if this is from plant or sheep wool?
1 answer-
As mentioned in the PROPERTIES section of the product page, this material is from sheep wool.
Helpful?
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