This product forms an initial 1:1 complex to thrombin, followed by a slower rate of aggregation relative to bovine proteins. For assay purposes, in QC, the interaction of heparin, thrombin, and antithrombin III is allowed to proceed at 25°C for exactly two minutes and the residual thrombin activity in the reaction is measured for calculation. A brief search of the literature shows that ATIII is a serine protease inhibitor that irreversibly inactivates thrombin, a serine protease, in a 1:1 complex. It is recommended to test in this application to determine if there is residual thrombin activity.
A2221
Antithrombin III aus Humanplasma
lyophilized powder, ≥95% (SDS-PAGE)
Synonym(e):
Faktor-Xa-Inhibitor, Heparin-Cofaktor
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25 μG
€ 186,00
125 μG
€ 662,00
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25 μG
€ 186,00
125 μG
€ 662,00
About This Item
Empfohlene Produkte
Biologische Quelle
human plasma
Qualitätsniveau
Assay
≥95% (SDS-PAGE)
Form
lyophilized powder
UniProt-Hinterlegungsnummer
Lagertemp.
−20°C
Angaben zum Gen
human ... SERPINC1(462)
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Biochem./physiol. Wirkung
Serinproteasehemmer; Inhibitor von Thrombin und aktiviertem Faktor X (Xa). Effektive Konzentration äquimolar zu Proteinase.
Verlinkung
Sollte nicht mit Thrombin-Antiserum verwechselt werden.
Einheitendefinition
Eine Einheit bildet einen Komplex und hemmt eine Einheit Rinderthrombin bei 25 °C, wobei eine 5-minütige Inkubation in Gegenwart von Heparin erfolgt.
Physikalische Form
Lyophilisiert aus 20 mM Tris, pH 8,0
Haftungsausschluss
NUR FÜR FORSCHUNGSZWECKE. Dieses Produkt unterliegt in Frankreich den Vorschriften für den Gebrauch zu wissenschaftlichen Zwecken, auch bei der Ein- und Ausfuhr (Artikel L 1211-1 Absatz 2 des französischen Gesundheitsgesetzes). Der Käufer (d. h. der Endverbraucher) ist verpflichtet, eine Einfuhrgenehmigung des französischen Forschungsministeriums gemäß Artikel L1245-5-1 II. des französischen Gesundheitsgesetzes einzuholen. Mit der Bestellung dieses Produkts bestätigen Sie, dass Sie über die entsprechende Einfuhrgenehmigung verfügen.
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Analysenzertifikate (COA)
Lot/Batch Number
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Kunden haben sich ebenfalls angesehen
Antithrombin activity is inhibited by acrolein and homocysteine thiolactone: Protection by cysteine.
Alejandro Gugliucci
Life sciences, 82(7-8), 413-418 (2008-01-22)
Conditions in which serum or tissue acrolein levels are high (e.g.: renal failure, heavy smoking, oxidative stress) are also associated with increased thrombogenicity. Another emerging cardiovascular risk factor is homocysteine, and its derivative, homocysteine thiolactone. Antithrombin is one of the
Yu-Fang Hsieh et al.
Biomedical materials (Bristol, England), 12(6), 065004-065004 (2017-10-06)
Biodegradable polymers have potential as a scaffold material for making small diameter artery bypass grafts. To resist thrombosis, maintain biocompatibility and enhance the remodeling of the grafts, it is crucial to modify polymer scaffolds so that the grafts have antithrombogenic
Xiaopeng Tang et al.
Cell research, 30(2), 119-132 (2019-12-08)
Coagulation balance is maintained through fine-tuned interactions among clotting factors, whose physiological concentrations vary substantially. In particular, the concentrations of coagulation proteases (pM to nM) are much lower than their natural inactivator antithrombin (AT, ~ 3 μM), suggesting the existence of other coordinators.
Hao Zhang et al.
Frontiers in cellular and infection microbiology, 11, 677902-677902 (2021-08-03)
Patients with sepsis may progress to acute respiratory distress syndrome (ARDS). Evidence of neutrophil extracellular traps (NETs) in sepsis-induced lung injury has been reported. However, the role of circulating NETs in the progression and thrombotic tendency of sepsis-induced lung injury
Protokolle
Thrombin is an endolytic serine protease that selectively cleaves the Arg–Gly bonds of fibrinogen to form fibrin and release fibrinopeptides A and B.
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Would incubation of bovine thrombin with antithrombin in 1:1 ratio in the presence of heparin for 5 min at 25 degrees Celsius, form irreversible thrombin-antithrombin complex and prevent thrombin's ability to degrade Tos-Gly-Pro-Arg-ANBA-IPA substrate?
1 answer-
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Dear, could you please provide me information what is the best way to dissolve product A2221 (Antithrombin III from human plasma)? Could I use distilled water? Would it be soluble at concentration 0.4 mg/ml? Thank you. Kind regards, Dusan
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This product is lyophilized from a solution containing 20 mM Tris, pH 8.0. For testing purposes, the material is reconstituted in the same buffer. The solubility of this product in water has not been validated. However, similar available products are listed as being water soluble.
Product SAE0102, lyophilized from PBS, pH 7.4., soluble in water at 0.1 mg/mL
Product 169756, lyophilized from 100 mM sodium citrate buffer, 20 mM Tris-HCl, pH 8.3, soluble in water on a lot specific basis, approximately 1mg/mL
Product 1040504, USP reference standard lyophilized from 0.05 M Tris, 0.15 M NaCl, pH 7.4, 2 mg/mL of trehalose, and 10 mg/mL of human albumin. - water soluble, no rate givenHelpful?
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What is Antithrombin III from human plasma, Product A2221, soluble in?
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The manufacturer has found it to be soluble in 50 mM Tris buffer with 150 mM sodium chloride. (No specific pH or concentration was provided.)
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the molecular weight for Antithrombin III from human plasma. Product A2221?
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The molecular weight of Antithrombin III from human plasma is approximately 53 kDa.
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The Antithrombin III from human plasma, Product A2221, vial appears to be empty. What should I do?
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What you are observing is normal for this product. Each vial has sub-milligram amounts in it (either 25 or 125 micrograms), which may not be visible. One should reconstitute the vial, and measure the absorbance at 280 nm to show that it does have protein present.
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What is the specific activity of Antithrombin III from human plasma?
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We do not have strict specifications for the specific activity of the A2221. It is typically in the range of 40-80 units per mg protein. The value is reported on the certificate of analysis for each lot.
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