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Merck

75259

Tris(2-carboxyethyl)phosphin -hydrochlorid

BioUltra, ≥98% (NMR)

Synonym(e):

TCEP

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Über diesen Artikel

Empirische Formel (Hill-System):
C9H15O6P · HCl
CAS-Nummer:
Molekulargewicht:
286.65
UNSPSC Code:
12352128
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
3724376

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InChI key

PBVAJRFEEOIAGW-UHFFFAOYSA-N

SMILES string

Cl[H].OC(=O)CCP(CCC(O)=O)CCC(O)=O

InChI

1S/C9H15O6P.ClH/c10-7(11)1-4-16(5-2-8(12)13)6-3-9(14)15;/h1-6H2,(H,10,11)(H,12,13)(H,14,15);1H

product line

BioUltra

assay

≥98% (NMR)

form

crystals

reaction suitability

reagent type: reductant

loss

≤1.0% loss on drying

pH

1.2-1.5

solubility

H2O: 1 M, clear, colorless (no residue)

anion traces

sulfate (SO42-): ≤50 mg/kg

cation traces

Al: ≤5 mg/kg, As: ≤0.05 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg

λ

0.1 M in H2O

UV absorption

λ: 260 nm Amax: 0.05, λ: 280 nm Amax: 0.03

storage temp.

2-8°C

Quality Level

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Dieser Artikel
68957C4706580560
assay

≥98% (NMR)

assay

97.5-102.5%

assay

-

assay

≥98% (elemental analysis)

Quality Level

300

Quality Level

100

Quality Level

300

Quality Level

100

product line

BioUltra

product line

BioUltra

product line

-

product line

-

form

crystals

form

powder

form

powder

form

crystalline solid

solubility

H2O: 1 M, clear, colorless (no residue)

solubility

H2O: soluble

solubility

H2O: 50 mg/mL

solubility

water: 5 mg/mL

anion traces

sulfate (SO42-): ≤50 mg/kg

anion traces

-

anion traces

-

anion traces

-

General description

Tris(2-carboxyethyl)-phosphinhydrochlorid (TCEP) ist ein starkes Reduktionsmittel, das in wässriger Lösung stabil ist.[1][2] Es reduziert Disulfide leicht, stöchiometrisch und mit hoher Spezifizität.[1] TCEP weist bei einem pH-Wert von 1,5 bis 9,0 eine reduzierende Wirkung auf und reduziert Dehydroascorbinsäure (DHA) bei einem pH-Wert von 4.[2]

Application

Tris(2-carboxyethyl)phosphinhydrochlorid wird wie folgt verwendet:
  • als Komponente von IRE1-Dialysepuffer, RNA-Spaltungspuffer und in einem In-vitro-Spaltungsassay von in vitro transkribierter RNA[3]
  • beim Reduzieren von Sulfhydrylgruppen, um die Gesamt-Thiol-Fraktion zu erhalten[4]
  • zum Reduzieren von Proteinproben[5]

Wasserlösliches Reagenz zur selektiven Reduktion von Disulfiden. Stabiler als DDT und nützlich für massenspektrometrische Anwendungen.

pictograms

Corrosion

signalword

Danger

hcodes

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Lagerklasse

8A - Combustible corrosive hazardous materials

wgk

WGK 1


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The kinase/RNase IRE1 is a key effector of the cellular response to endoplasmic reticulum stress. The RNase activity of IRE1 can be measured in cells or in the test tube. Here we describe a protocol for the in vitro cleavage
Yasunori Sato et al.
Biological & pharmaceutical bulletin, 33(3), 364-369 (2010-03-02)
Ascorbic acid (AA) has a strong anti-oxidant function evident as its ability to scavenge superoxide radicals in vitro. Moreover, AA is an essential ingredient for post-translational proline hydroxylation of collagen molecules. Dehydroascorbic acid (DHA), the oxidized form of AA, is
Lena Munzel et al.
Autophagy, 17(6), 1458-1478 (2020-06-10)
Coupling of Atg8 to phosphatidylethanolamine is crucial for the expansion of the crescent-shaped phagophore during cargo engulfment. Atg21, a PtdIns3P-binding beta-propeller protein, scaffolds Atg8 and its E3-like complex Atg12-Atg5-Atg16 during lipidation. The crystal structure of Atg21, in complex with the
Maria João Correia et al.
Antioxidants (Basel, Switzerland), 10(9) (2021-09-29)
We hypothesized that an interplay between aryl hydrocarbon receptor (AhR) and cysteine-related thiolome at the kidney cortex underlies the mechanisms of (mal)adaptation to chronic intermittent hypoxia (CIH), promoting arterial hypertension (HTN). Using a rat model of CIH-HTN, we investigated the
Lucas B Harrington et al.
Molecular cell, 79(3), 416-424 (2020-07-10)
CRISPR-Cas12c/d proteins share limited homology with Cas12a and Cas9 bacterial CRISPR RNA (crRNA)-guided nucleases used widely for genome editing and DNA detection. However, Cas12c (C2c3)- and Cas12d (CasY)-catalyzed DNA cleavage and genome editing activities have not been directly observed. We

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Questions

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  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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