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Sigma-Aldrich

Goat Anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, Alkaline Phosphatase, Species Adsorbed

Chemicon®, from goat

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About This Item

UNSPSC-Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

Biologische Quelle

goat

Qualitätsniveau

Konjugat

alkaline phosphatase conjugate

Antikörperform

purified antibody

Antikörper-Produkttyp

secondary antibodies

Klon

polyclonal

Speziesreaktivität

mouse

Hersteller/Markenname

Chemicon®

Methode(n)

ELISA: suitable

Versandbedingung

wet ice

Posttranslationale Modifikation Target

unmodified

Verwandte Kategorien

Allgemeine Beschreibung

IgG is the most abundant immunoglobulin comprising two heavy chains and two light chains. Each molecule has two antigen binding sites. IgG has 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). IgM constitutes about 10% of serum immunoglobulins. IgM (with IgD) is the major immunoglobulin expressed on the surface of B cells. Monomeric IgA constitutes 5-15 % of the serum immunoglobulins whereas dimeric IgA is localized to mucosa surfaces.

Spezifität

Reacts with heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA. Absorbed for human sera and purified human paraproteins. Minimal cross reactivity with human immunoglobulins.
The antibody reacts with the heavy and light chains of mouse IgM, IgG1, IgG2a, IgG2b, IgG3 and IgA as demonstrated by ELISA and flow cytometry. Minimal cross reactivity with human immunoglobulins.

Immunogen

Epitope: Mouse IgM, IgG, IgA
Prepared from pepsin digest of mouse Ig (H+L).

Anwendung

Research Category
Sekundär- & Kontrollantikörper
Research Sub Category
Fragmentspezifische Sekundärantikörper
ELISA: 1:2,000-1:4,000.

Optimal working dilutions must be determined by the end user.
Goat anti-Mouse IgM+IgG+IgA Antibody, F(ab′)2, Alkaline Phosphatase, Species Adsorbed is an antibody against Mouse IgM+IgG+IgA for use in ELISA.

Physikalische Form

Gel filtration chromatography
Purified by gel filtration chromatography of pepsin digested antibody. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.

Lagerung und Haltbarkeit

Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

Rechtliche Hinweise

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1


Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

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RNA (New York, N.Y.), 23(7), 1028-1034 (2017-04-09)
Nonsense-mediated mRNA decay (NMD) is a eukaryotic mRNA degradation pathway involved in surveillance and post-transcriptional regulation, and executed by the concerted action of several trans-acting factors. The SMG1 kinase is an essential NMD factor in metazoans and is associated with
Structural analysis of the yeast Dhh1-Pat1 complex reveals how Dhh1 engages Pat1, Edc3 and RNA in mutually exclusive interactions.
Sharif, H; Ozgur, S; Sharma, K; Basquin, C; Urlaub, H; Conti, E
Nucleic Acids Research null

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