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Merck

P2921

Sigma-Aldrich

Monoclonal Anti-Protein A antibody produced in mouse

clone SPA-27, ascites fluid

Synonym(e):

Monoclonal Anti-Protein A

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100 μL
€ 542,00
0.2 ML
€ 701,00

€ 542,00


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100 μL
€ 542,00
0.2 ML
€ 701,00

About This Item

UNSPSC-Code:
12352203
NACRES:
NA.46

€ 542,00


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Biologische Quelle

mouse

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

ascites fluid

Antikörper-Produkttyp

primary antibodies

Klon

SPA-27, monoclonal

Enthält

15 mM sodium azide

Methode(n)

immunocytochemistry: suitable
indirect ELISA: 1:20,000
western blot: suitable

Isotyp

IgG1

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Protein A is a 42 kDa single chain polypeptide isolated from the cell wall of Staphylococcus aureus Cowan I strain.

Spezifität

Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Immunogen

protein A from Cowan I strain of Staphy­lococcus aureus.

Anwendung

Monoclonal Anti-Protein A antibody produced in mouse has been used in enzyme-linked immunosorbent assay (ELISA). It has also been used in western blot and dot blot analyses.

Biochem./physiol. Wirkung

Protein A is considered as a universal reagent in biochemistry and immunology, due to its affinity for the Fc region of many mammalian immunoglobulins. It is used for different applications such as purification of immunoglobulins by affinity chromatography, cell surface studies, radioimmunoassay (RIA), enzyme immunoassay (EIA), immunoprecipitations and many other procedures. It can be used in these methods either in its native form or conjugated to various markers.

Physikalische Form

Monoclonal Anti-Protein A antibody produced in mouse is provided as ascites fluid with 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

nwg

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Analysenzertifikate (COA)

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Die Dokumentenbibliothek aufrufen

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Steindl F, et al.
Journal of Immunological Methods, 217(1-2), 143-151 (1998)
Sandra K Schuller et al.
eLife, 9 (2020-11-17)
The yeast THO complex is recruited to active genes and interacts with the RNA-dependent ATPase Sub2 to facilitate the formation of mature export-competent messenger ribonucleoprotein particles and to prevent the co-transcriptional formation of RNA:DNA-hybrid-containing structures. How THO-containing complexes function at
Shan Huang et al.
Frontiers in molecular biosciences, 7, 581095-581095 (2021-01-12)
Hog1 is a mitogen-activated protein kinase in yeast that primarily regulates cellular responses to hyperosmolarity stress. In this study, we have examined the potential involvement of Hog1 in mediating cellular responses to DNA damaging agents. We find that treatment of
Fabien Bonneau et al.
Genes & development, 37(11-12), 505-517 (2023-07-03)
Messenger RNAs (mRNAs) are at the center of the central dogma of molecular biology. In eukaryotic cells, these long ribonucleic acid polymers do not exist as naked transcripts; rather, they associate with mRNA-binding proteins to form messenger ribonucleoprotein (mRNP) complexes.
Stephanie Marroquin et al.
Infection and immunity, 87(5) (2019-03-06)
Numerous factors have, to date, been identified as playing a role in the regulation of Agr activity in Staphylococcus aureus, including transcription factors, antisense RNAs, and host elements. Herein we investigated the product of SAUSA300_1984 (termed MroQ), a transmembrane Abi-domain/M79

Questions

  1. I would like to know the concentration (mg/mL) of the anti-proA antibody. Thanks!

    1 answer
    1. The concentration of this antibody is reported on a lot specific basis. The product specification range is 6 - 12 mg/mL. A brief review of the lot history shows a typical range of 8 - 10 mg/mL. Please see the link below to review a sample or lot specific certificate:
      https://www.sigmaaldrich.com/product/sigma/p2921#product-documentation

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