06-1363

Anti-SRp30a (SF2) Antibody

from rabbit

• Synonym(e): serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2, P33 subunit, SR splicing factor 1, splicing factor 2, splicing factor, arginine/serine-rich 1, Alternative-splicing factor 1
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: polyclonal
• Speziesreaktivität: mouse, rat, human
• Methode(n): immunocytochemistry: suitable; western blot: suitable
• NCBI-Hinterlegungsnummer: NP_001071634
• UniProt-Hinterlegungsnummer: Q07955
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... SRSF1(6426)

Beschreibung

Allgemeine Beschreibung

SRp30a, also known as SF2, ASF-1 (alternative splicing factor 1), is a member of the highly conserved family of SR proteins, a group of serine/arginine-rich domain-containing proteins that play a critical role in regulated and constitutive splicing of precursor mRNA. The SR family of proteins is characterized as having one to two RNA recognition motifs located in the n-terminal sequence, and an RS domain within the C-terminus that is rich in serine/arginine dipeptide repeats. ASF-1 has an inhibitory role in exon skipping, in order to maintain splicing accuracy and to monitor alternative splicing. Studies have shown that ASF-1 plays a role in the regulation of caspase-9 alternative splicing as well as being a critical component for ceramide to function as a mediator of alternative splicing for the same caspase.

Immunogen

Linear peptide corresponding to human SRp30a.

Anwendung

Research Category
Epigenetik & nukleäre Funktionen

Research Sub Category
RNA-Stoffwechsel & Bindungsproteine

Immunocytochemistry Analysis:
1:500 dilution from a representative lot detected SRp30a in NIH/3T3 cells.

Use Anti-SRp30a (SF2) Antibody (rabbit polyclonal antibody) validated in WB, ICC to detect SRp30a (SF2) also known as serine/arginine-rich splicing factor 1, pre-mRNA-splicing factor SF2- P33 subunit, SR splicing factor 1.

Qualität

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1:500 dilution of this antibody detected SRp30a on 10 µg of NIH/3T3 cell lysate.

Zielbeschreibung

~40 kDa observed. An uncharacterized band at ~ 50 kDa has been observed in some lysates.

Physikalische Form

Protein A

Format: Purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
NIH/3T3 cell lysate

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable