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S8504

Sigma-Aldrich

Sulfatase from Patella vulgata (keyhole limpet)

Type IV, essentially salt-free, lyophilized powder, ≥10 units/mg solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

limpet (Patella vulgata)

Quality Level

100

type

Type IV

form

essentially salt-free, lyophilized powder

specific activity

≥10 units/mg solid

foreign activity

β-Glucuronidase ≥750 units/mg solid

storage temp.

−20°C

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Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kosuke Yusa
Nature protocols, 8(10), 2061-2078 (2013-09-28)
I report here a detailed protocol for seamless genome editing using the piggyBac transposon in human pluripotent stem cells (hPSCs). Recent advances in custom endonucleases have enabled us to routinely perform genome editing in hPSCs. Conventional approaches use the Cre/loxP
S Saha et al.
Pharmacological research, 65(6), 592-602 (2012-03-01)
Epicatechin is a widely consumed dietary flavonoid and there is substantial evidence that it contributes to the health benefits reported for flavanol-rich cocoa products including dark chocolate. Numerous reports have described the appearance of epicatechin and epicatechin phase-2 conjugates (sulfates
Mengxian Long et al.
Journal of bacteriology, 193(15), 4041-4041 (2011-05-31)
Pseudomonas sp. strain S9 was originally isolated from mangrove soil in Xiamen, China. It is an aerobic bacterium which shows extracellular arylsulfatase activity. Here, we describe the 4.8-Mb draft genome sequence of Pseudomonas sp. S9, which exhibits novel cysteine-type sulfatases.
Fatemeh Moheimani et al.
Atherosclerosis, 223(1), 144-151 (2012-06-05)
People with diabetes have an elevated risk of atherosclerosis. The accumulation of lipid within macrophage cells in the artery wall is believed to arise via the uptake and subsequent processing of modified low-density lipoproteins (LDL) via the endo-lysosomal system. In
P R Warman et al.
Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes, 45(7), 633-638 (2010-08-31)
The study was undertaken to determine the impact of high-metal composts on the activities of four soil enzymes. High concentrations of metal salts (Cr, Cu, Ni or a Co-Mo-Pb combination) were added to feedstocks during the thermophilic stage of composting.
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Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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