This enzyme is lyophilized from a solution containing buffer salts and a stabilizer. The product may be reconsituted at 1 mg/mL in water. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/412/974/g7646dat.pdf
G7646
β-Glucuronidase from Escherichia coli
Type VII-A, lyophilized powder, 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Synonym(s):
β-D-Glucuronide glucuronosohydrolase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-606-8
MDL number:
Specific activity:
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
Pricing and availability is not currently available.
type
Type VII-A
Quality Segment
form
lyophilized powder
specific activity
5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay)
mol wt
290 kDa
composition
Protein, ~25% biuret
storage temp.
−20°C
Application
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine
Effective in the hydrolysis of steroid glucuronides.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis
Analysis Note
Contains buffer salts and stabilizer.
Other Notes
One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at the pH 6.8 (30 min assay).
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 5,000,000-20,000,000 units/g protein, pH 6.8 (30 min assay) | specific activity ≥20,000 units/mg protein | specific activity ≥300,000 units/g solid | specific activity ≥60,000 units/mL |
| form lyophilized powder | form lyophilized powder | form partially purified powder | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt 290 kDa | mol wt 290 kDa | mol wt - | mol wt - |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 300 |
| composition Protein, ~25% biuret | composition , ~25% | composition - | composition - |
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signalword
Danger
Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
wgk
WGK 1
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It is recommended to resuspend the product in which buffer?
1 answer-
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Is BSA or other protein components included in the overall percentage listed on the CoA for the product G7646, which is β-Glucuronidase from Escherichia coli (CAS 9001-45-0)?
1 answer-
No. BSA or other protein sources are added to the product except Potassium Phosphate and Sucrose. Although this is a fermentation product, there may be other protein residues in it in a negligible amount. The product is tested by activity, residual protein contaminants are not tested. The protein percentage is the total protein (tested by Biuret method).
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