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N3877

Sigma-Aldrich

Potassium 4-nitrophenyl sulfate

sulfatase substrate, chromogenic, ≥98% (TLC), powder

Synonym(s):

4-Nitrophenyl sulfate potassium salt

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About This Item

Linear Formula:
NO2C6H4OSO2OK
CAS Number:
6217-68-1
Molecular Weight:
257.26
Beilstein:
3786392
EC Number:
228-288-5
MDL number:
MFCD00007466
UNSPSC Code:
12352204
PubChem Substance ID:
24897664
NACRES:
NA.32
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Product Name

Potassium 4-nitrophenyl sulfate, sulfatase substrate

Quality Level

200

Assay

≥98% (TLC)

form

powder

mp

246-250 °C (lit.)

solubility

water: 50 mg/mL, clear, colorless to very faintly greenish-yellow (to Very Light Yellow)

shipped in

wet ice

storage temp.

−20°C

SMILES string

[K+].[O-][N+](=O)c1ccc(OS([O-])(=O)=O)cc1

InChI

1S/C6H5NO6S.K/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;/h1-4H,(H,10,11,12);/q;+1/p-1

InChI key

BITVAZYUWRLLCN-UHFFFAOYSA-M

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Application

Potassium 4-nitrophenyl sulfate has been used:
  • as a substrate to measure arylsulfatase activity in cell-free coelomic fluid[1]
  • as a substrate for p-nitrophenyl glycoside-based enzyme assay[2]
  • to inhibit arylsulfatase[3]
  • as an inorganic analog of organic aryl ester sulphate[4]

Substrates

Chromogenic sulfatase substrate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000394988
0000394989
0000386318
0000372975
0000368692

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J O Falkinham
International journal of systematic bacteriology, 40(1), 66-70 (1990-01-01)
A rapid (3-h) arylsulfatase assay for cell suspensions of mycobacteria, in which p-nitrophenyl sulfate is used as the substrate, was developed. Arylsulfatase activity was found in cell suspensions of representative strains of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium scrofulaceum grown
M P Kung et al.
Endocrinology, 122(4), 1195-1200 (1988-04-01)
Subcellular preparations from rat liver, brain, and kidney and from human liver were tested for their ability to desulfate T3 sulfate (T3SO4). Activity was found associated with the microsomal fraction: rat liver was the most active, hydrolyzing 76 pmol/min.mg protein
H X Zhang et al.
Drug metabolism and disposition: the biological fate of chemicals, 19(2), 473-477 (1991-03-01)
Although numerous previous reports have characterized the mammalian biotransformation of the organophosphorus insecticides parathion and methyl parathion, questions still remain regarding the toxicological significance of certain metabolic pathways in vivo. The present study utilized rat liver perfusions in order to
Proteomic profiles reveal age-related changes in coelomic fluid of sea urchin species with different life spans
Bodnar A
Experimental Gerontology, 48(5), 525-530 (2013)
N Sakuma-Sawada et al.
Research communications in molecular pathology and pharmacology, 97(2), 131-138 (1997-08-01)
Hepatic uptake of the sulfate conjugate of p-nitrophenol (p-NPsul) has been studied. Uptake clearance of p-NPsul by isolated rat hepatocytes was dependent on p-NPsul concentration, suggesting carrier-mediated transport. The uptake of p-NPsul by isolated rat hepatocytes was inhibited by acetaminophen
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