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T5574

Sigma-Aldrich

Terrific Broth

Liquid microbial growth medium

Synonym(s):

TB medium, Tartoff – Hobbs medium

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.85

grade

for molecular biology

description

Liquid Medium

sterility

sterile; 0.2 μm filtered

form

liquid

technique(s)

microbiological culture: suitable

application(s)

microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli
nonselective for coliforms

General description

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Application

Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.

Features and Benefits

Terrific Broth liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Components

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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