If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
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About This Item
UNSPSC Code:
41106514
Biological source:
mouse adipose tissue
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast
Pricing and availability is not currently available.
biological source
mouse adipose tissue
packaging
tube of 5 μg 85011425-DNA-5UG, pkg of vial of cells 85011425-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: PRV, VSV (Indiana), HSV
Biochem/physiol Actions
Mouse C3H/An connective tissue
Subclone of parental strain L, established by W R Earle in 1940. One of the first to be established in continuous culture. The L strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+.
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split semi-confluent cultures seeding at 5x1,000 to 2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].
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Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Line cells?
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How many Cell Line cells are in the vial?
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Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
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What passage are my Cell Line cells?
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If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
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How do I handle Cell Line frozen cells upon arrival?
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Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
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What medium should I use when I thaw the Cell Line cells?
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The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
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Are my Cell Line cells mycoplasma tested?
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ECACC routinely tests all manufactured cell banks for mycoplasma.
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Are the Cell Line cells tested for viral pathogens?
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HPA Cultures does not perform any viral testing on the cell lines.
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