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Sanger Arrayed Whole Genome Lentiviral CRISPR Library

Name: Sanger Arrayed Whole Genome Lentiviral CRISPR Library
Brand: SIGMA

Mouse, Virus Format

Synonym(s):

Arrayed CRISPR library, Sanger CRISPR library

About This Item

UNSPSC Code:

41105904

Concentration:

1x10⁶ VP/ml (via p24 assay)

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Properties

Quality Level

200

packaging

pkg of 10 μL (384-well plate)

concentration

1x10⁶ VP/ml (via p24 assay)

application(s)

CRISPR

shipped in

dry ice

storage temp.

−70°C

Description

General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.

Application

Functional Genomics/Screening /Target Validation

Features and Benefits

Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
Simplify the workflow with puromycin selection
Illuminate CRISPR-expressing cells with BFP

Additional Features

Better, not bigger: Two optimized clones per mouse gene reduces the time, cost, and scale of screening experiments
Ready-to-screen: Clones are arrayed in a robotics-friendly 384-well format for high throughput screening
Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here

Packaging

384 well plates

Physical form

10μl of lentivirus at ≥10⁶ particles/ml in ~120×384 well plates

Other Notes

Additional libraries are available:
Human, Glycerol Format
Mouse, Glycerol Format
Human, Virus Format

Lentivirus Transduction Particles of gRNA-only lenti-based vectors. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones

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This product is for R&D use only, not for drug, household, or other uses. Lentivirus manufacturing is achieved by using 2nd generation packaging plasmids on a 3rd generation lenti-based vector. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

Compare Similar Items

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Show Differences

1 of 1

This Item	HSANGERV	MSANGERG	HKCRISPR
Sigma-Aldrich MSANGERV Sanger Arrayed Whole Genome Lentiviral CRISPR Library	Sigma-Aldrich HSANGERV Sanger Arrayed Whole Genome Lentiviral CRISPR Library	Sigma-Aldrich MSANGERG Sanger Arrayed Whole Genome Lentiviral CRISPR Library	Sigma-Aldrich HKCRISPR Human Kinase Lentiviral CRISPR Pool
concentration 1x10⁶ VP/ml (via p24 assay)	concentration 1x10⁶ VP/ml (via p24 assay)	concentration -	concentration 5x10⁸ VP/ml (via p24 assay)
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
application(s) CRISPR	application(s) CRISPR	application(s) CRISPR	application(s) CRISPR
packaging pkg of 10 μL (384-well plate)	packaging pkg of 10 μL (384-well plate)	packaging pkg of 50 μL (96-well plate)	packaging pkg of 8x25 μL (vials)

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

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Protocols

Sanger Sequencing Steps & Method

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Séquençage Sanger : étapes et méthode

Découvrez les étapes de la méthode de Sanger ou séquençage par terminaison de chaîne, le fonctionnement du séquençage de l'ADN, et apprenez à lire précisément les résultats d'un séquençage Sanger pour vos propres recherches.

Articles

Successful Transduction Using Lentivirus

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Sanger Whole Genome CRISPR Libraries

Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.

Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries.

Metzakopian, E. et al

Scientific Reports, 7 (2017)

Global Trade Item Number

SKU	GTIN
MSANGERV-1EA	04061826266328

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