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10567752001

Roche

Alkaline Phosphatase

EIA grade, from calf intestine

Synonym(s):

AP, Alkaline Phosphatase

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Pack SizeSKUAvailabilityPrice
1.5 mL
Please contact Customer Service for Availability
$1,250.00

About This Item

UNSPSC Code:
12352204
EC Number:
3.1.3.1 (BRENDA, IUBMB)
NACRES:
NA.54
Specific activity:
~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)
Biological source:
bovine (calf) intestine

$1,250.00

Please contact Customer Service for Availability
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biological source

bovine (calf) intestine

form

solution

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

packaging

pkg of 1.5 mL (15 mg)

manufacturer/tradename

Roche

optimum pH

9.8

pH range

9.0-11.0

shipped in

wet ice

General description

Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
Amino groups: 8-16 moles/mole enzyme

Application

Alkaline phosphatase can be coupled to other proteins via its amino groups. The enzyme solution can be used directly for conjugation without prior dialysis.

Physical form

Solution, ready-to-use, 10mg/ml

Preparation Note

Storage conditions (working solution): The diluted enzyme (1:10 in 3 M NaCl) will give a stability of 6 months at 4 °C.
1:10 dilution in water will give stability for 1 week at 4°C.
Do not freeze the diluted or undiluted enzyme, because freezing will destroy enzyme activity.
Store at 2 to 8 °C. (Do not freeze)

Analysis Note

Purity: single peak (HPLC)

Other Notes

Purification procedure
AP from intestine is a dimeric glycoprotein, it is not known, whether the carbohydrate part is separated during purification procedure. Content of amino groupsIn the literature the carbohydrate content is 2 to 20 percent so one should find approx. 70 amino groups/mol.
In addition one researcher determined the amino groups and found 50 to 60 lysins/mol.-SH groups in AP
No information in literature about -SH groups in AP.

Carbohydrate structure
Results of investigation of the carbohydrate structure of several lots of alkaline phosphatase are available.

NH2- and carbohydrate estimation
Results of NH2- and carbohydrate estimation are available.
For life science research only. Not for use in diagnostic procedures.
Unit Conversion: Approx. 4.35 U (EIA grade AP) [37 °C, 4-NPP as substrate, diethanolamine as buffer, pH 9.8] = 1 U [+25 °C, 4-NPP as substrate, glycine as buffer, pH 10.5]
1.0 U [+37 °C, QC assay conditions] = 0.73 U [+30 °C, QC assay conditions] = 0.48 U [+25 °C, QC assay conditions]

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This Item
P5521APMB-ROP7923
Alkaline Phosphatase EIA grade, from calf intestine

Roche

10567752001

Alkaline Phosphatase

Phosphatase, Alkaline from bovine intestinal mucosa ≥2,000 DEA units/mg protein

Sigma-Aldrich

P5521

Phosphatase, Alkaline from bovine intestinal mucosa

Alkaline Phosphatase solution, from bovine (calf) intestine, 2 units/μg protein, optimum pH 7.5-9.5

Roche

APMB-RO

Alkaline Phosphatase

Phosphatase, Alkaline from bovine intestinal mucosa buffered aqueous glycerol solution, ≥4,000 DEA units/mg protein

Sigma-Aldrich

P7923

Phosphatase, Alkaline from bovine intestinal mucosa

specific activity

~3,000 U/mg enzyme protein (+37°C, 4-nitrophenyl phosphate substrate, diethanolamine as buffer)

specific activity

≥2,000 DEA units/mg protein

specific activity

2 units/μg protein

specific activity

≥4,000 DEA units/mg protein

biological source

bovine (calf) intestine

biological source

-

biological source

bovine (calf) intestine

biological source

-

form

solution

form

aqueous solution

form

solution

form

buffered aqueous glycerol solution

shipped in

wet ice

shipped in

-

shipped in

wet ice

shipped in

-

optimum pH

9.8

optimum pH

-

optimum pH

7.5-9.5

optimum pH

-

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

Roche

manufacturer/tradename

-

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Certificates of Analysis (COA)

Lot/Batch Number
43968633
32666825
32666824
23811244
17248336

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Protocols

Alkaline Phosphatase Protocol

Alkaline phosphatase (AP) is a non-specific phosphomonoester hydrolase that catalyzes the hydrolysis of a wide variety of organic monophosphates.

Yupeng Li et al.
Chemical research in toxicology, 34(4), 992-1003 (2021-03-12)
The tobacco-specific nitrosamines N'-nitrosonornicotine (NNN) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) are considered to be two of the most important carcinogens in unburned tobacco and its smoke. They readily cause tumors in laboratory animals and are classified as "carcinogenic to humans" by the
Kathryn H Morelli et al.
Nature neuroscience, 26(1), 27-38 (2022-12-13)
Huntington's disease (HD) is a fatal, dominantly inherited neurodegenerative disorder caused by CAG trinucleotide expansion in exon 1 of the huntingtin (HTT) gene. Since the reduction of pathogenic mutant HTT messenger RNA is therapeutic, we developed a mutant allele-sensitive CAGEX

Global Trade Item Number

SKUGTIN
1056775200104061838250544