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M6250

2-Mercaptoethanol

≥99.0%

Synonym(s):

β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol

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About This Item

Linear Formula:
HSCH2CH2OH
CAS Number:
60-24-2
Molecular Weight:
78.13
UNSPSC Code:
12352100
NACRES:
NA.22
PubChem Substance ID:
57654402
EC Number:
200-464-6
Beilstein/REAXYS Number:
773648
MDL number:
MFCD00004890
Assay:
≥99.0%
Bp:
157 °C (lit.)
Vapor pressure:
1 mmHg ( 20 °C)
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vapor density

2.69 (vs air)

Quality Level

200

vapor pressure

1 mmHg ( 20 °C)

assay

≥99.0%

form

liquid

expl. lim.

18 %

concentration

14.3 M (pure liquid)

refractive index

n20/D 1.500 (lit.)

pH

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

storage temp.

2-8°C

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

General description

2-mercaptoethanol is a thiol compound, commonly used as a reducing agent in organic reactions.

Application

2-mercaptoethanol is widely used for retarding oxidation of biological compounds in solution.
BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

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This Item
137151PHR9274M3148
2-Mercaptoethanol ≥99.0%

Sigma-Aldrich

M6250

2-Mercaptoethanol

2-Mercaptoethanol EMPROVE® EVOLVE

SAFC

137151

2-Mercaptoethanol

2-Mercaptoethanol certified reference material, TraceCERT®

Supelco

PHR9274

2-Mercaptoethanol

2-Mercaptoethanol Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sigma-Aldrich

M3148

2-Mercaptoethanol

assay

≥99.0%

assay

-

assay

-

assay

99% (GC/titration)

Quality Level

200

Quality Level

400

Quality Level

300

Quality Level

200

form

liquid

form

liquid

form

-

form

liquid

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

density

1.114 g/mL at 25 °C (lit.)

bp

157 °C (lit.)

bp

157 °C (lit.)

bp

157 °C (lit.)

bp

157 °C (lit.)

pH

4.5-6 (20 °C, 500 g/L)

pH

-

pH

-

pH

4.5-6 (20 °C, 500 g/L)

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Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

target_organs

Liver,Heart

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

165.2 °F - closed cup

flash_point_c

74 °C - closed cup

ppe

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT4347
BCCP0675
BCCM9818
SHBS4916
BCCM8003

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Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines.
Asfari M, et al.
Endocrinology, 130(1), 167-178 (1992)
Silver stain for proteins in polyacrylamide gels: a modified procedure with enhanced uniform sensitivity.
Morrissey JH.
Analytical Biochemistry, 117(2), 307-310 (1981)
Manching Ku et al.
PLoS genetics, 4(10), e1000242-e1000242 (2008-11-01)
In embryonic stem (ES) cells, bivalent chromatin domains with overlapping repressive (H3 lysine 27 tri-methylation) and activating (H3 lysine 4 tri-methylation) histone modifications mark the promoters of more than 2,000 genes. To gain insight into the structure and function of
View All Related Papers

Global Trade Item Number

SKUGTIN
M6250-100ML04061837253232
M6250-900UL04061837253300
M6250-500ML04061837253294
M6250-1L04061837253256
M6250-10ML04061837253249
M6250-2.5L04061837253263
M6250-250ML04061837253270

Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Can this bME be use in cell culture?

    1 answer

    1. This product has not been assessed for suitability in cell culture. Alternative product M3148, 2-Mercaptoethanol, is labeled as suitable for cell culture.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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