LC/MS Analysis of Peptides on Discovery^® BIO Wide Pore C18, Effect of TFA Concentration on MS Signal

column

Discovery^® BIO Wide Pore C18, 15 cm x 2.1 mm I.D., 3 μm particles (567202-U)

mobile phase

[A]: aqueous component (25 mM formic acid, 0.01% TFA or 0.1% TFA); [B] (50:50) water:acetonitrile containing same ionic additives as aq. component

flow rate

0.20 mL/min

column temp.

ambient

detector

(+)ESI

injection

1-3 μL

sample

RP Peptide Performance Standard (Alberta Peptide Inst., Cat. No. RPS-P0010)