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Merck

HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4

HPLC Analysis of Protein Standards (1) on BIOshell A400 Protein C4 application for HPLC

材料

分析柱

產品號碼
描述
訂價

66825-U

BIOshell A400 Protein C4, 3.4 μm HPLC Column

3.4 μm particle size, L × I.D. 10 cm × 2.1 mm

標準

產品號碼
描述
訂價

G8511

β 半乳糖苷酶 来源于大肠杆菌

For use as a marker in SDS-PAGE

L1254

L-乳酸脱氢酶 来源于兔肌肉

Type XI, lyophilized powder, 600-1,200 units/mg protein

A7642

白蛋白 来源于鸡蛋白

For use as a marker in SDS-PAGE

L6876

Lysozyme 来源于鸡蛋白

powder or granules, ≥90 %, ≥39,000 units/mg protein

C0755

伴清蛋白 来源于鸡蛋白

Substantially iron-free

H0138

人结合珠蛋白，表型 1-1

98-100%, essentially salt-free, lyophilized powder

T2327

胰蛋白酶抑制剂 来源于大豆

BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

防護柱套

產品號碼
描述
訂價

66830-U

BIOshell A400 Protein C4, 3.4 μm Guard Cartridge

3.4 μm particle size, L × I.D. 0.5 cm × 2.1 mm, pkg of 3 ea

CONDITIONS

column

BIOshell A400 Protein C4, 10 cm X 2.1 mm, 3.4 μm particles (66825-U)

mobile phase

(A) 75:25, [0.1% TFA in water]:[0.1% TFA in acetonitrile] ; (B) 25:75, [0.1% TFA in water]:[0.1% TFA in acetonitrile]

gradient

25 to 65% B in 8 min

flow rate

0.3 mL/min

pressure

1430 psi (99 bar)

detector

UV, 215 nm

injection

1 μL

sample

protein mixture, g/L ea: lysozyme, 0.1; haptoglobulin, 0.3; conalbumin, 0.3; trypsin inhibitor, 0.3; glutamate dehydrogenase, 0.8; beta-galactosidase, 0.7; ovalbumin, 0.8; lactate dehydrogenase, 0.4

描述

分析報告

This application demonstrates the utility of the BIOshell A400 Protein C4 column for the reversed-phase chromatography of proteins from a variety of sources and of different molecular weights.

法律資訊

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Chromatogram Search

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