In this assay, the activity of L-Lactic Dehydrogenase is determined by observing the decrease in absorbance at 340 nm for NADH, rather than the formation of NAD+. The method utilizes a continuous spectrophotometric rate determination to monitor the absorbance decrease at 340 nm, corresponding to NADH absorbance.
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推薦產品
生物源
rabbit muscle
品質等級
種類
Type XI
形狀
lyophilized powder
比活性
600-1,200 units/mg protein
分子量
140 kDa
成份
protein, 90-100%
儲存條件
(Keep container tightly closed in a dry and well-ventilated place)
技術
activity assay: suitable
顏色
white
異物活動
pyruvate kinase, myokinase, malic dehydrogenase, glutamic-pyruvic transaminase, glutamic-oxalacetic transaminase and α-glycerophosphate dehydrogenase ≤0.01%
儲存溫度
−20°C
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一般說明
研究领域： 细胞信号传导
乳酸脱氢酶（LDH）的总分子量为140kDa，由4个亚基组成，称为M亚基（肌肉）和H亚基（心脏）。这些亚基可以以5种组合（M4、M3H1、M2H2、MH3和H4）中的任何一种进行混合。 骨骼肌含有LDH，其主要是M4，含有少量M3H和微量的H2H2。 H和M亚基的分子量非常相似，但氨基酸组成十分不同。兔肌肉LDH在pH5.0的乙酸盐-氯化物中可解离为二聚体物质（MW = ~70kDa），该解离是可逆的。Biochemistry, 13, 3527-3531 (1974). 氧化乙醛酸和乳酸。
等电点：8.4-8.6
最适pH值：7.5.
等电点：8.4-8.6
最适pH值：7.5.
應用
生化／生理作用
其还可催化其他L-2-羟基单羧酸的氧化。
肌型乳酸脱氢酶(LDH)参与多种代谢途径，是无氧糖酵解的必需活性成分。乳酸脱氢酶活性可被抗坏血酸抑制。乳酸脱氢酶可从NADH中再生烟酰胺腺嘌呤二核苷酸(NAD+)，工业上可用于生产聚乳酸。[4]在无氧条件下，LDH 参与发酵反应，催化丙酮酸生成乳酸，并将烟酰胺腺嘌呤二核苷酸 (NADH) 氧化为NAD+。因此，LDH 可介导NAD+基础无氧糖酵解途径。通过这一途径，LDH 有助于在缺氧的情况下维持细胞的生理和生化功能。
單位定義
在pH 7.5、37 °C条件下，一单位酶每分钟可将1.0 μmole丙酮酸还原成L-乳酸。
分析報告
通过缩二脲法测定的蛋白质。
抑制劑
抗體
酶
產品號碼
描述
訂價
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
個人防護裝備
Eyeshields, Gloves, type N95 (US)
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
For use as a marker in SDS-PAGE; Albumin from chicken egg white, For use as a marker in SDS-PAGE; L-Lactic Dehydrogenase from rabbit muscle, Type XI, lyophilized powder, 600-1,200 units/mg protein
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In the assay, the UV-Vis absorbance at 340 nm is always showing negative. If lactate continues to form over time, shouldn't the absorbance become more positive?
1 answer-
Helpful?
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What are products that only have the quality release date on the COA and do not have an expiration or retest date?
1 answer-
Certain products are not included in the retest or expiration date programs as there is no indication to suggest that they are unstable. These products will only display the Release Date on the certificate of analysis, with no stated Retest, Expiration, or Use-by Date. It is advised to handle these products according to the defined conditions outlined in our product literature and website product descriptions. It is recommended for end users to regularly inspect these products to ensure they perform as expected. For such products, our standard warranty of 1 year from the date of shipment applies. For more information on our warranty, please refer to the terms and conditions of sale.
Helpful?
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is the substrate given for the enzyme lactate or lactic form, and may I know why it is L-lactic dehydrogenase and not L-lactate dehydrogenase?
1 answer-
The terms tend to be used interchangeably. Lactate is the anion of the conjugate base lactic acid.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/216/153/l1254enz.pdfHelpful?
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