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568223-U

Supelco

Discovery® BIO Wide Pore C18 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm

同義詞:

大孔C18 HPLC色谱柱

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52
暫時無法取得訂價和供貨情況

產品名稱

Discovery® BIO 大孔 C18 高效液相色谱柱, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

材料

stainless steel column

agency

suitable for USP L1

產品線

Discovery®

特點

endcapped

製造商／商標名

Discovery®

包裝

1 ea of

標籤範圍

9.2% Carbon loading

參數

0-70 °C temperature
400 bar pressure (5801 psi)

技術

HPLC: suitable
LC/MS: suitable

長度 × 內徑

25 cm × 4.6 mm

表面積

100 m2/g

表面覆盖率

3.6 μmol/m2

基質

silica particle platform
fully porous particle

基質活性組

C18 (octadecyl) phase

粒徑

5 μm

孔徑

300 Å

工作pH值

2-8

分離技術

reversed phase

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一般說明

RP-HPLC 上获得的多肽谱图可提供许多关于蛋白质结构、稳定性和纯度等有价值的信息。为达到预期效果，RP-HPLC 色谱柱必须能够分离样品中高百分比的多肽。多肽含量越高，获得的信息越有用。Discovery BIO 大孔 C18 柱可从酶解物中获得分离度很高的 RP-HPLC 多肽谱图。对 Discovery BIO 大孔系列产品所采用的硅胶和键合相化学性质的改进，增加了色谱柱的柱效并减少了拖尾，因而提高分离度。另一个优点是流动相中不需要 TFA 即可分析多肽，从而增强了 LC-MS 信号。

應用


  • 采用梯度动力学图比较表面多孔、全多孔和整体反相色谱柱在蛋白质生物药物分离中的动力学性能。：本研究对Discovery® BIO大孔C18 HPLC柱等不同类型HPLC反相色谱柱进行了详细的动力学性能比较。研究重点介绍了色谱柱分离复杂蛋白质生物制药方面的效率和分辨率，使其成为分析化学家在生物制药分析中的宝贵工具（Jaag et al., 2022）。

  • 使用全多孔和部分多孔固定相组合对胰蛋白酶消化物进行RP-LC x RP-LC分析。：本文研究了使用配备全多孔和部分多孔固定相（包括BIO大孔C18 HPLC色谱柱）的反相液相色谱（RP-LC）分析胰蛋白酶消化物。研究结果证明，它在蛋白质组学应用中具有稳健性和高分辨率，能够实现精确的肽图分析和蛋白质表征（Mondello et al., 2010）。

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法律資訊

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

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分析證明 (COA)

Lot/Batch Number
294894-06
294894-05
294894-04
294894-03
294894-02

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Hannah F Speer et al.
Journal of animal science, 98(9) (2020-08-28)
This study assessed the relative bioavailability of guanidinoacetic acid (GAA) in cattle. Seven ruminally cannulated Holstein steers (initial body weight of 280 kg) were used in an experiment with a 5 × 5 Latin square design; the two additional steers

相關內容

DiscoveryBIO Wide Pore C18

DiscoveryBIO Wide Pore C18 products offered by Sigma-Aldrich.

DiscoveryBIO 寬孔 C18

DiscoveryBIO Wide Pore C18 產品由 Sigma-Aldrich 提供。

Chromatograms

application for HPLC

Questions

1–2 of 2 Questions  

  1. What is the method for rewetting a column that may have dried as a result of the end cap being loose or not being used for an extended period of time?

    1 answer

    1. Reverse Phase Column Rehydration Method:
      1. The column should ideally not contain any buffer. If it does, thoroughly flush the column with water to remove the buffer.
      2. Flush the column with 100% acetonitrile, starting at a flow rate of 0.3-0.5 ml/minute. Slowly increase the flow rate to 1 ml/minute, flushing for approximately 30 minutes, as long as the pressure does not excessively increase.
      3. Equilibrate the column with the mobile phase specified on the Certificate of Analysis (CofA) of the column, and run a test mix similar to what was used to test the column.
      4. If the performance is unsatisfactory, repeat the method, increasing the flushing with acetonitrile for one hour.
      5. Repeat the test. If the performance is still not as expected, the column may have degraded.

      Helpful?

  2. WHIGH SOLVENT USED FOR WASHING OF COLUMN

    1 answer

    1. See below for a general cleaning protocol:
      1. Reverse the orientation of the column
      2. Flush for a few minutes, or >1 column volume, with a 20% solution in water, of the organic used in the analysis, such as methanol or acetonitrile. This is to remove any residual buffer salts.
      3. Flush for approximately 30 mins, or >10 column volumes, with 100% of the organic solvent used in the analysis.
      4. The column can be stored with this organic solvent until ready for use.
      5. Before reusing the column, flush again, as per step 2.

      Helpful?

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