Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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產品名稱
Ascentis® Express RP-Amide 2.7μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
材料
stainless steel column
品質等級
agency
suitable for USP L60
產品線
Ascentis®
特點
endcapped
製造商／商標名
Ascentis®
包裝
1 ea of
參數
60 °C temp. range
600 bar max. pressure (9000 psi)
技術
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
長度 × 內徑
10 cm × 2.1 mm
表面積
135 m2/g
雜質
<5 ppm metals
基質
Fused-Core® particle platform
superficially porous particle
基質活性組
amide, alkyl phase
粒徑
2.7 μm
孔徑
90 Å
工作pH值範圍
2-9
應用
food and beverages
分離技術
reversed phase
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法律資訊
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.
應用
產品號碼
描述
訂價
相關產品
防護柱套
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
從最近期的版本中選擇一個：
分析證明 (COA)
Lot/Batch Number
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文章
A significantly improved HPLC-fluorescence method for DMB-NANA and -NGNA, and application of this method to compare 2 candidate biosimilar therapeutic proteins to their respective RMs.
Use QuEChERS methods to analyze samples of milk for veterinary drugs.
Innovative particle technology and RP-Amide bonding chemistry enhance chromatography performance for improved peak shape and resolution.
創新的微粒技術與 RP-Amide 鍵合化學技術可增強色譜性能，改善峰形與解析度。
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相關內容
QuEChERS method simplifies sample cleanup for pesticides analysis in food and agricultural samples.
QuEChERS 方法簡化了食品和農產品樣品中農藥分析的樣品清理工作。
Chromatograms
application for HPLCapplication for HPLCapplication for HPLCapplication for HPLC顯示更多-
What is the Department of Transportation shipping information for this product?
1 answer-
Helpful?
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Can Ascentis® Express HPLC Columns be used for LC-MS?
1 answer-
Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Is there anything special I need to do to my HPLC system to use Ascentis Express?
1 answer-
Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)
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Can I use Ascentis Express on a UHPLC system?
1 answer-
Yes. Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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What is the carbon load for Ascentis Express RP-Amide?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for Ascentis Express RP-Amide is 3.0 micromoles/meter-squared.
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Can I use Ascentis Express on any type of HPLC system?
1 answer-
Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems. Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
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What flow rate should I use with Ascentis® Express HPLC Columns?
1 answer-
Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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