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53914-U

Supelco

Ascentis® Express 90 Å RP-Amide (2.7 μm) HPLC Columns

L × I.D. 15 cm × 2.1 mm, HPLC Column

同義詞:

Core-shell (SPP) Fused Core polar embedded HPLC column

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About This Item

分類程式碼代碼:
41115700
eCl@ss:
32110501
NACRES:
SB.52
暫時無法取得訂價和供貨情況

產品名稱

Ascentis® Express RP-Amide 2.7μm HPLC 色谱柱, 2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

材料

stainless steel column

品質等級

100

agency

suitable for USP L60

產品線

Ascentis®

特點

endcapped

製造商／商標名

Ascentis®

包裝

1 ea of

參數

60 °C temp. range
600 bar max. pressure (9000 psi)

技術

HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

長度 × 內徑

15 cm × 2.1 mm

表面積

135 m2/g

雜質

<5 ppm metals

基質

Fused-Core® particle platform
superficially porous particle

基質活性組

amide, alkyl phase

粒徑

2.7 μm

孔徑

90 Å

工作pH值範圍

2-9

應用

food and beverages

分離技術

reversed phase

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相關類別

應用

使用Ascentis® Express RP-Amide（2.7 μm）对防晒霜进行UHPLC分析

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

從最近期的版本中選擇一個：

分析證明 (COA)

Lot/Batch Number
USQX002235
USQX002234
USQX002233
USQX002232
USQX002230

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Katia Arena et al.
Journal of separation science, 43(9-10), 1781-1789 (2020-04-03)
Olea europaea, meaning "European olive," is a small tree belonging to the family Oleaceae, occurring in the Mediterranean Basin. Olive oil is an essential component of a balanced diet because of its nutritional value. Among micronutrients, phenolic compounds did show important beneficial effects for human health.
Michael T Furlong et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 926, 36-41 (2013-04-02)
Clopidogrel is prescribed for the treatment of Acute Coronary Syndrome and recent myocardial infarction, recent stroke, or established peripheral arterial disease. A sensitive and reliable high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay was developed and validated to enable reliable
Erzsébet Oláh et al.
Journal of chromatography. A, 1217(23), 3642-3653 (2010-04-23)
Today sub-2 microm packed columns are very popular to conduct fast chromatographic separations. The mass-transfer resistance depends on the particle size but some practical limits exist not to reach the theoretically expected plate height and mass-transfer resistance. Another approach applies
Martin Mzik et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1020, 85-89 (2016-04-01)
A simple, rapid and sensitive method based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been developed and validated for the quantitative determination in rat plasma of a new candidate for AD treatment, namely PC 48 (a 7-MEOTA-donepezil like compound) in
Maria A Khalikova et al.
Talanta, 130, 433-441 (2014-08-28)
The presented work describes the development of a simple, fast and effective on-line SPE-UHPLC-UV/vis method using fused core particle columns for extraction, separation and quantitative analysis of the nine illegal dyes, most frequently found in chilli-containing spices. The red dyes
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Use QuEChERS methods to analyze samples of milk for veterinary drugs.

Ascentis® Express RP-Amide HPLC Columns

Innovative particle technology and RP-Amide bonding chemistry enhance chromatography performance for improved peak shape and resolution.

Ascentis® Express RP-Amide HPLC 色譜柱

創新的微粒技術與 RP-Amide 鍵合化學技術可增強色譜性能，改善峰形與解析度。

Synthetic Cannabinoid Analysis

A large variety of cannabinoid drug standards, along with an HPLC method with the Supelco Ascentis® Express RP-Amide column, are presented in this article.

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Questions

1–10 of 10 Questions  

  1. What is the pressure limit for the product 53914-U?

    1 answer

    1. The pressure limit for the product Ascentis Express RP-Amide 2.7um HPLC column (53914-U) is 9000 PSI.

      Helpful?

  2. How can I measure my instrument bandwidth (IBW) and determine what Ascentis® Express HPLC Columns can be used with minimal efficiency loss created by too much internal instrument volume?

    1 answer

    1. The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Is there anything special I need to do to my HPLC system to use Ascentis Express?

    1 answer

    1. Nothing special is required to use Ascentis Express HPLC columns. To obtain the full benefits of Ascentis Express, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Systems for Ascentis Express Columns (T407102)

      Helpful?

  5. Can I use Ascentis Express on a UHPLC system?

    1 answer

    1. Yes.  Ascentis Express columns are packed in a way making them suitable for these ultra high pressure instruments.  In fact, Ascentis Express outperforms sub-2 μm micron columns on many applications since Ascentis Express provides the benefits of sub-2 μm particles but at much lower back pressure.  These benefits include the capability of providing fast HPLC and higher resolution chromatography.  The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell.  A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.

      Helpful?

  6. What flow rate should I use with Ascentis® Express HPLC Columns?

    1 answer

    1. Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize Ascentis Express column efficiency. The suggested starting point for flow rate for Ascentis Express columns: 1.6 mL/min for 4.6 mm ID;  0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.

      Helpful?

  7. Can I use Ascentis Express on any type of HPLC system?

    1 answer

    1. Ascentis Express HPLC columns are capable of use on standard HPLC systems as well as UHPLC systems.  Columns are packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.

      Helpful?

  8. Do I need special fittings and tubing to connect Ascentis® Express HPLC Columns?

    1 answer

    1. While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.

      Helpful?

  9. Can Ascentis® Express HPLC Columns be used for LC-MS?

    1 answer

    1. Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of Ascentis Express columns for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.

      Helpful?

  10. What is the carbon load for Ascentis Express RP-Amide?

    1 answer

    1. Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles.  The carbon coverage for Ascentis Express RP-Amide is 3.0 micromoles/meter-squared.

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