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Merck
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重要文件

G005723

LC/MS Analysis of Polypeptide Drugs on Ascentis® Express RP-Amide

application for HPLC

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About This Item

技術

HPLC: suitable

測試參數

column: Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
mobile phase: [A] 10 mM ammonium acetate in water, pH 4.2; [B] 10 mM ammonium acetate in 90:10 (v/v) acetonitrile:water, pH 4.7
gradient: held at 20% B for 0.1 min; 20 to 80% B in 2.9 min; 80 to 20% B in 0.01 min; held at 20% B for 0.99 min
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: TOF, ESI(+), m/z 100-2500
injection: 3 μL
sample: 1 μL/mL in 10mM ammonium formate, 80:20, acetonitrile:water

適合性

application for HPLC

應用

life science and biopharma

分析報告

This application demonstrates the suitability of the Ascentis Express RP Amide for the analysis of polypeptide drugs.

法律資訊

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

描述

    Oxytocin
    [deamino-Cys1, D-Arg8]-Vasopressin acetate salt hydrate

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分析證明 (COA)

Lot/Batch Number

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