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重要文件

Y1376

Sigma-Aldrich

酵母营养缺陷型培养基补充剂

without leucine

同義詞:

合成酵母生长培养基

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About This Item

MDL號碼:
分類程式碼代碼:
12000000
NACRES:
ND.02

應用

由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。

成分

除非另有说明,否则补充剂包含以下所有成分:
氨基酸:除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质:腺嘌呤(18 mg/L),肌醇(76 mg/L),对氨基苯甲酸(8 mg/L),尿嘧啶(76 mg / L)。

其他說明

氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。

數量

1 l 培养基将补充 1.6 g。

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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文章

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基因工程可以大規模地表達和分離用於研究目的的重組蛋白質。

條款

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Yeast culture techniques: Model systems for eukaryotic studies with liquid media or agar plate growth.

我們的科學家團隊在所有研究領域都有豐富的經驗,包括生命科學、材料科學、化學合成、色譜、分析等.

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