Autoclaving is not recommended with glucose/dextrose according to the product information sheet for this product. However, autoclaving can be performed without the sugar, and filter sterilized glucose/dextrose can be added afterward.
推薦產品
等級
Molecular Biology
品質等級
無菌
non-sterile
形狀
powder
技術
microbe id | utilization test: suitable
microbiological culture: suitable
pH值
5.2-5.6(0.67% solution)
應用
food and beverages
microbiology
儲存溫度
room temp
適合性
nonselective for Candida spp.
nonselective for Pichia spp.
nonselective for Saccharomyces spp.
nonselective for Zygosaccharomyces spp.
yeasts
一般說明
酵母氮基是培养野生型酵母公认的理想选择。目前可确知，氨基酸存在会妨碍选择性标记物使用。因此，这种培养基制剂之中不含氨基酸，可用于酵母人工染色体的克隆和操作。[1]
酵母菌氮基是酵母菌生长的重要培养基。 这种富含营养的微生物肉汤含有氮、维生素、微量元素和盐。
應用
不含氨基酸的酵母氮基已用于：
适用于根据氨基酸和碳的需要对酵母菌进行分类。 可能需要添加氨基酸和碳源(通常是葡萄糖)。
成分
组成部分，含量(μg/L，除非注明)
氮源：
硫酸铵，5.0 g/L
维生素：
生物素，2.0
泛酸钙，400
叶酸，2.0
肌醇，2.0 mg / L
烟酸，400
对氨基苯甲酸，200
盐酸吡哆醇，400
核黄素，200
盐酸硫胺素，400
微量元素：
硼酸，500
硫酸铜，40
碘化钾，100
氯化铁，200
硫酸锰，400
钼酸钠，200
硫酸锌，400
盐类：
磷酸二氢钾，1.0g/L
硫酸镁，0.5g/L
氯化钠，0.1g/L
氯化钙，0.1g/L
氮源：
硫酸铵，5.0 g/L
维生素：
生物素，2.0
泛酸钙，400
叶酸，2.0
肌醇，2.0 mg / L
烟酸，400
对氨基苯甲酸，200
盐酸吡哆醇，400
核黄素，200
盐酸硫胺素，400
微量元素：
硼酸，500
硫酸铜，40
碘化钾，100
氯化铁，200
硫酸锰，400
钼酸钠，200
硫酸锌，400
盐类：
磷酸二氢钾，1.0g/L
硫酸镁，0.5g/L
氯化钠，0.1g/L
氯化钙，0.1g/L
準備報告
1.将6.7 g酵母氮碱、5 g葡萄糖或等量的其他碳水化合物和5-10 mg所需氨基酸溶解在100 ml蒸馏水中，制备10x储备溶液。
2.必要时可加温溶解，然后过滤灭菌。
2-8°C储存10x储备溶液。使用前，在无菌条件下用无菌蒸馏水1:10稀释。
2.必要时可加温溶解，然后过滤灭菌。
2-8°C储存10x储备溶液。使用前，在无菌条件下用无菌蒸馏水1:10稀释。
相關產品
產品號碼
描述
訂價
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Why is autoclaving this not recommended?
1 answer-
Helpful?
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Can this be autoclaved, or only filter sterilized?
1 answer-
It is not recommended to autoclave Product Y0626 Yeast Nitrogen Base Without Amino Acids. If preparing an bacteriological agar, then the agar should be autoclaved separately for 15 minutes only. Please follow the instructions listed on the product detail page:
1. Prepare a 10× stock solution by suspending 6.7 g of yeast nitrogen base, 5 g of glucose or an equivalent amount of other carbohydrate, and 5-10 mg of the desired amino acid in 100 ml of distilled water.
2. Warm if necessary to solubilize and sterilize by filtration.
Store 10× stock solution at 2-8 °C. To use, dilute 1:10 with sterile distilled water under aspetic conditions.Helpful?
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